ArticleScientific reports2017
CRISPR/Cas9-mediated genome editing efficiently creates specific mutations at multiple loci using one sgRNA in Brassica napus.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 96 papers.
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Who cites it
96 citing papers in PubMed.
- Research progress in gene editing of Brassicaceae crops.Biodesign research · 2026Review
- Transport engineering as a strategy to realize rapeseed's potential as a protein-rich food.Pest management science · 2026Review
- Establishment of CRISPR/Cas9 Genome Editing inJournal of fungi (Basel, Switzerland) · 2026Article
- Turbocharging crop breeding with integrated biotechnology for a climate-resilient future.Journal of integrative plant biology · 2026Review
- Genome editing beyond transgenes: DNA-free technologies in crop improvement.Frontiers in plant science · 2026Review
- Speed breeding enhances crop resilience and productivity in a changing climate.Molecular breeding : new strategies in plant improvement · 2025Review
- Review
- Unlocking genetic potential: a review of the role of CRISPR/Cas technologies in rapeseed improvement.Stress biology · 2025Review
- Applications of CRISPR/Cas tools in improving stress tolerance inFrontiers in plant science · 2025Review
- High-temperature stress in strawberry: understanding physiological, biochemical and molecular responses.Planta · 2024Review
- Agrobacterium tumefaciens-Mediated Plant Transformation: A Review.Molecular biotechnology · 2024Review
- Review
- Plasma and synovial fluid extracellular vesicles display altered microRNA profiles in horses with naturally occurring post-traumatic osteoarthritis: an exploratory study.Journal of the American Veterinary Medical Association · 2024Article
- Review
- Metabolome and transcriptome analyses reveal changes of rapeseed in response to ABA signal during early seedling development.BMC plant biology · 2024Article
- Harnessing the potential of mutation breeding, CRISPR genome editing, and beyond for sustainable agriculture.Functional & integrative genomics · 2024Review
- Breeding rice for yield improvement through CRISPR/Cas9 genome editing method: current technologies and examples.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- Putting CRISPR-Cas system in action: a golden window for efficient and precise genome editing for crop improvement.GM crops & food · 2023Review
- The current scenario and future perspectives of transgenic oilseed mustard by CRISPR-Cas9.Molecular biology reports · 2023Review
- Comparative transcriptome profiling reveals the multiple levels of crosstalk in phytohormone networks in Brassica napus.Plant biotechnology journal · 2023Article
36 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CRISPR/Cas9 is a valuable tool for both basic and applied research that has been widely applied to different plant species. Nonetheless, a systematical assessment of the efficiency of this method is not available for the allotetraploid Brassica napus-an important oilseed crop. In this study, we examined the mutation efficiency of the CRISPR/Cas9 method for 12 genes and also determined the pattern, specificity and heritability of these gene modifications in B. napus. The average mutation frequency for a single-gene targeted sgRNA in the T0 generation is 65.3%. For paralogous genes located in conserved regions that were targeted by sgRNAs, we observed mutation frequencies that ranged from 27.6% to 96.6%. Homozygotes were readily found in T0 plants. A total of 48.2% of the gene mutations, including homozygotes, bi-alleles, and heterozygotes were stably inherited as classic Mendelian alleles in the next generation (T1) without any new mutations or reversions. Moreover, no mutation was found in the putative off-target sites among the examined T0 plants. Collectively, our results demonstrate that CRISPR/Cas9 is an efficient tool for creating targeted genome modifications at multiple loci that are stable and inheritable in B. napus. These findings open many doors for biotechnological applications in oilseed crops.
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