ArticleThe Journal of cell biology1986
Potassium-dependent assembly of coated pits: new coated pits form as planar clathrin lattices.
Article in The Journal of cell biology, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- IVSA-based siRNA targeted delivery system as a universal strategy for the treatment of EGFR-positive cancer.Science China. Life sciences · 2026Article
- Laminar organization of molecular complexes in a clathrin coat revealed by nanoscale protein colocalization.Structure (London, England : 1993) · 2025Article
- Vitamin C-Dependent Uptake of Non-Heme Iron by Enterocytes, Its Impact on Erythropoiesis and Redox Capacity of Human Erythrocytes.Antioxidants (Basel, Switzerland) · 2024Review
- Mechanistic-Based Classification of Endocytosis-Related Inhibitors: Does It Aid in Assigning Drugs against SARS-CoV-2?Viruses · 2023Review
- Actin polymerization promotes invagination of flat clathrin-coated lattices in mammalian cells by pushing at lattice edges.Nature communications · 2022Article
- De novo endocytic clathrin coats develop curvature at early stages of their formation.Developmental cell · 2021Article
- Evolving models for assembling and shaping clathrin-coated pits.The Journal of cell biology · 2020Review
- Clathrin-adaptor ratio and membrane tension regulate the flat-to-curved transition of the clathrin coat during endocytosis.Nature communications · 2018Article
- Dileucine-like motifs in the C-terminal tail of connexin32 control its endocytosis and assembly into gap junctions.Journal of cell science · 2018Article
- Vacuolation Activity and Intracellular Trafficking of ArtB, the Binding Subunit of an AB5 Toxin Produced by Salmonella enterica Serovar Typhi.Infection and immunity · 2017Article
- Combined sodium ion sensitivity in agonist binding and internalization of vasopressin V1b receptors.Scientific reports · 2016Article
- A new strategy for specific imaging of neural cells based on peptide-conjugated gold nanoclusters.International journal of nanomedicine · 2015Article
- Flat clathrin lattices: stable features of the plasma membrane.Molecular biology of the cell · 2014Article
- Membrane fluctuations destabilize clathrin protein lattice order.Biophysical journal · 2014Article
- Identification of genes affecting vacuole membrane fragmentation in Saccharomyces cerevisiae.PloS one · 2013Article
- Identification of yeast genes involved in k homeostasis: loss of membrane traffic genes affects k uptake.G3 (Bethesda, Md.) · 2011Article
- Adaptor protein 2 regulates receptor-mediated endocytosis and cyst formation in Giardia lamblia.The Biochemical journal · 2010Article
- The use of inhibitors to study endocytic pathways of gene carriers: optimization and pitfalls.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- Epidermal growth factor receptor translocation to the mitochondria: regulation and effect.The Journal of biological chemistry · 2009Article
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3 authors.
Funding
Abstract
Previous studies have shown that when human fibroblasts are depleted of intracellular K+, coated pits disappear from the cell surface and the receptor-mediated endocytosis of low density lipoprotein (LDL) is inhibited. We have now used the K+ depletion protocol to study several aspects of coated pit function. First, since coated pits rapidly form when K+-depleted fibroblasts are incubated in the presence of 10 mM KCl, we studied the sequence of assembly of coated pits as visualized in carbon-platinum replicas of inner membrane surfaces from cells that had been incubated in the presence of K+ for various times. New coated pits initially appeared as planar clathrin lattices that increased in size by the formation of polygons at the margin of the lattice. Once the lattice reached a critical size it invaginated to form coated vesicles. Second, we determined that LDL-ferritin can induce clustering of LDL receptors over noncoated membrane on the surface of K+-depleted fibroblasts; however, when these cells are subsequently incubated in the presence of K+, these clusters become associated with newly formed coated pits and are internalized. Finally, we determined that K+ depletion inhibits the assembly of coated pits, but that existing coated pits in K+-depleted cells are able to internalize LDL. These results suggest that the clathrin lattice of coated pits is actively involved in membrane shape change during endocytosis and that the structural proteins of the lattice are cyclically assembled and disassembled in the process.
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