ArticleMaterials (Basel, Switzerland)2014
Field Effect Transistor Biosensor Using Antigen Binding Fragment for Detecting Tumor Marker in Human Serum.
Article in Materials (Basel, Switzerland), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 83 citations in OpenAlex.
- Recent Advances on Sensor Technologies for the Monitoring of Tumor Markers.Molecules (Basel, Switzerland) · 2026Review
- Plasmonic Single-Molecule Affinity Detection at 10Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Emerging Dual-Gate FET Sensor Paradigm for Ultra-Low Concentration Cortisol Detection in Complex Bioenvironments.Biosensors · 2025Article
- Electric Field Cycling of Physisorbed Antibodies Reduces Biolayer Polarization Dispersion.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Graphene-based field-effect transistors for biosensing: where is the field heading to?Analytical and bioanalytical chemistry · 2024Review
- Biosensors Based on Ion-Sensitive Field-Effect Transistors for HLA and MICA Antibody Detection in Kidney Transplantation.Molecules (Basel, Switzerland) · 2022Article
- Smartphone-Based Platforms for Clinical Detections in Lung-Cancer-Related Exhaled Breath Biomarkers: A Review.Biosensors · 2022Review
- Modeling of Quasi-Static Floating-Gate Transistor Biosensors.ACS sensors · 2021Article
- Combination of Aptamer Amplifier and Antigen-Binding Fragment Probe as a Novel Strategy to Improve Detection Limit of Silicon Nanowire Field-Effect Transistor Immunosensors.Sensors (Basel, Switzerland) · 2021Article
- Article
- A Reliable BioFET Immunosensor for Detection of p53 Tumour Suppressor in Physiological-Like Environment.Sensors (Basel, Switzerland) · 2020Article
- Chemically Crosslinked Bispecific Antibodies for Cancer Therapy: Breaking from the Structural Restrictions of the Genetic Fusion Approach.International journal of molecular sciences · 2020Article
- Microfluidic opportunities in printed electrolyte-gated transistor biosensors.Biomicrofluidics · 2020Article
- Field-Effect Transistor Biosensors for Biomedical Applications: Recent Advances and Future Prospects.Sensors (Basel, Switzerland) · 2019Review
- Signal Enhancement of Silicon Nanowire Field-Effect Transistor Immunosensors by RNA Aptamer.ACS omega · 2019Article
- Glycan-immobilized dual-channel field effect transistor biosensor for the rapid identification of pandemic influenza viral particles.Scientific reports · 2019Article
- Hybrid Silicon Nanowire Devices and Their Functional Diversity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Review
- Detection of Alpha-Fetoprotein in Hepatocellular Carcinoma Patient Plasma with Graphene Field-Effect Transistor.Sensors (Basel, Switzerland) · 2018Article
- [The application of silicon nanowires field-effect transistor biosensor in medicine].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2017Review
- Recent advances in electrochemical biosensors based on graphene two-dimensional nanomaterials.Biosensors & bioelectronics · 2016Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Detection of tumor markers is important for cancer diagnosis. Field-effect transistors (FETs) are a promising method for the label-free detection of trace amounts of biomolecules. However, detection of electrically charged proteins using antibody-immobilized FETs is limited by ionic screening by the large probe molecules adsorbed to the transistor gate surface, reducing sensor responsiveness. Here, we investigated the effect of probe molecule size on the detection of a tumor marker, α-fetoprotein (AFP) using a FET biosensor. We demonstrated that the small receptor antigen binding fragment (Fab), immobilized on a sensing surface as small as 2-3 nm, offers a higher degree of sensitivity and a wider concentration range (100 pg/mL-1 μg/mL) for the FET detection of AFP in buffer solution, compared to the whole antibody. Therefore, the use of a small Fab probe molecule instead of a whole antibody is shown to be effective for improving the sensitivity of AFP detection in FET biosensors. Furthermore, we also demonstrated that a Fab-immobilized FET subjected to a blocking treatment, to avoid non-specific interactions, could sensitively and selectively detect AFP in human serum.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.