ArticleDevelopmental cell2017
The Helicase Aquarius/EMB-4 Is Required to Overcome Intronic Barriers to Allow Nuclear RNAi Pathways to Heritably Silence Transcription.
Article in Developmental cell, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 73 citations in OpenAlex.
- TurboID proximity labeling reveals protein interaction networks for germline Argonautes.bioRxiv : the preprint server for biology · 2026Article
- TONSL suppresses polymerase theta-dependent tandem duplications through chromatin-guided repair.Nature communications · 2026Article
- piRNAs safeguard splicing and RNA fidelity during heat shock.bioRxiv : the preprint server for biology · 2025Article
- Nuclear Argonaute HRDE-1 Maintains Chromatin-Independent Transcriptional Silencing through a Transgenerational Small RNA Feedback Loop.bioRxiv : the preprint server for biology · 2025Article
- A unified framework governing the establishment and maintenance of transgenerational epigenetic inheritance.Genetics · 2025Article
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- Reduced insulin/IGF-1 signalling upregulates two anti-viral immune pathways, decreases viral load and increases survival under viral infection in C. elegans.GeroScience · 2024Article
- Two H3K23 histone methyltransferases, SET-32 and SET-21, function synergistically to promote nuclear RNAi-mediated transgenerational epigenetic inheritance inbioRxiv : the preprint server for biology · 2024Article
- The intron binding protein EMB-4 is an opposite regulator of cold and high temperature tolerance inPNAS nexus · 2024Article
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- Sensitized piRNA reporter identifies multiple RNA processing factors involved in piRNA-mediated gene silencing.Genetics · 2023Article
- Sensitized piRNA reporter identifies multiple RNA processing factors involved in piRNA-mediated gene silencing.bioRxiv : the preprint server for biology · 2023Article
- The conserved helicase ZNFX-1 memorializes silenced RNAs in perinuclear condensates.Nature cell biology · 2022Article
- Review
- Article
- Secondary structures in RNA synthesis, splicing and translation.Computational and structural biotechnology journal · 2022Review
- Small RNAs in epigenetic inheritance: from mechanisms to trait transmission.FEBS letters · 2021Review
- Antagonistic cotranscriptional regulation through ARGONAUTE1 and the THO/TREX complex orchestratesProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Introns control stochastic allele expression bias.Nature communications · 2021Article
- Ageing as "early-life inertia": Disentangling life-history trade-offs along a lifetime of an individual.Evolution letters · 2021Article
Corrections and comments
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Authors and funding
17 authors at 7 institutions in 4 countries.
Funding
Abstract
Small RNAs play a crucial role in genome defense against transposable elements and guide Argonaute proteins to nascent RNA transcripts to induce co-transcriptional gene silencing. However, the molecular basis of this process remains unknown. Here, we identify the conserved RNA helicase Aquarius/EMB-4 as a direct and essential link between small RNA pathways and the transcriptional machinery in Caenorhabditis elegans. Aquarius physically interacts with the germline Argonaute HRDE-1. Aquarius is required to initiate small-RNA-induced heritable gene silencing. HRDE-1 and Aquarius silence overlapping sets of genes and transposable elements. Surprisingly, removal of introns from a target gene abolishes the requirement for Aquarius, but not HRDE-1, for small RNA-dependent gene silencing. We conclude that Aquarius allows small RNA pathways to compete for access to nascent transcripts undergoing co-transcriptional splicing in order to detect and silence transposable elements. Thus, Aquarius and HRDE-1 act as gatekeepers coordinating gene expression and genome defense.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.