ArticlePLoS genetics2017
MicroRNA-275 targets sarco/endoplasmic reticulum Ca2+ adenosine triphosphatase (SERCA) to control key functions in the mosquito gut.
Article in PLoS genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
- Host serum-circulating flavivirus NS1 co-opts mosquito miRNA processing to suppress early immune defense.Nature communications · 2026Article
- Serine Hydroxymethyltransferase Modulates Midgut Physiology inBiomolecules · 2025Article
- Sensory regulation of meal sorting in Aedes aegypti mosquitoes.Scientific reports · 2024Article
- Analysis ofbioRxiv : the preprint server for biology · 2024Article
- Differential gene expression and microRNA profile in corpora allata-corpora cardiaca of Aedes aegypti mosquitoes with weak juvenile hormone signalling.BMC genomics · 2024Article
- Analysis ofFrontiers in cellular and infection microbiology · 2024Article
- A glimpse into the world of microRNAs and their putative roles in hard ticks.Frontiers in cell and developmental biology · 2024Review
- The influence of the larval microbiome on susceptibility to Zika virus is mosquito genotype-dependent.PLoS pathogens · 2023Article
- MicroRNA-989 targets 5-hydroxytryptamine receptor1 to regulate ovarian development and eggs production in Culex pipiens pallens.Parasites & vectors · 2023Article
- Notch Signaling in Insect Development: A Simple Pathway with Diverse Functions.International journal of molecular sciences · 2023Review
- Variable microbiomes between mosquito lines are maintained across different environments.PLoS neglected tropical diseases · 2023Article
- miR-275/305 cluster is essential for maintaining energy metabolic homeostasis by the insulin signaling pathway in Bactrocera dorsalis.PLoS genetics · 2022Article
- Dynamics of Small Non-coding RNA Profiles and the Intestinal Microbiome of High and Low Weight Chickens.Frontiers in microbiology · 2022Article
- Hormonal regulation of microRNA expression dynamics in the gut of the yellow fever mosquitoRNA biology · 2021Article
- Juvenile hormone regulation of microRNAs is mediated by E75 in the Dengue vector mosquitoProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Human blood microRNA hsa-miR-21-5p induces vitellogenin in the mosquito Aedes aegypti.Communications biology · 2021Article
- Paratransgenic manipulation of a tsetse microRNA alters the physiological homeostasis of the fly's midgut environment.PLoS pathogens · 2021Article
- Microbial interactions in the mosquito gut determine Serratia colonization and blood-feeding propensity.The ISME journal · 2021Article
- The roles of the gut microbiota-miRNA interaction in the host pathophysiology.Molecular medicine (Cambridge, Mass.) · 2020Review
- Dynamic miRNA-mRNA interactions coordinate gene expression in adult Anopheles gambiae.PLoS genetics · 2020Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
The yellow fever mosquito Aedes aegypti is the major vector of arboviruses, causing numerous devastating human diseases, such as dengue and yellow fevers, Chikungunya and Zika. Female mosquitoes need vertebrate blood for egg development, and repeated cycles of blood feeding are tightly linked to pathogen transmission. The mosquito's posterior midgut (gut) is involved in blood digestion and also serves as an entry point for pathogens. Thus, the mosquito gut is an important tissue to investigate. The miRNA aae-miR-275 (miR-275) has been shown to be required for normal blood digestion in the female mosquito; however, the mechanism of its action has remained unknown. Here, we demonstrate that miR-275 directly targets and positively regulates sarco/endoplasmic reticulum Ca2+ adenosine triphosphatase, which is implicated in active transport of Ca2+ from the cytosol to the sarco/endoplasmic reticulum. We utilized a combination of the gut-specific yeast transcription activator protein Gal4/upstream activating sequence (Gal4/UAS) system and miRNA Tough Decoy technology to deplete the endogenous level of miR-275 in guts of transgenic mosquitoes. This gut-specific reduction of miR-275 post blood meal decreased SERCA mRNA and protein levels of the digestive enzyme late trypsin. It also resulted in a significant reduction of gut microbiota. Moreover, the decrease of miR-275 and SERCA correlated with defects in the Notch signaling pathway and assembly of the gut actin cytoskeleton. The adverse phenotypes caused by miR-275 silencing were rescued by injections of miR-275 mimic. Thus, we have discovered that miR-275 directly targets SERCA, and the maintenance of its level is critical for multiple gut functions in mosquitoes.
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