ArticleParasites & vectors2017
Detecting and characterizing mixed infections with genetic variants of Anaplasma phagocytophilum in roe deer (Capreolus capreolus) by developing an ankA cluster-specific nested PCR.
Article in Parasites & vectors, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- Diversity ofAnimals : an open access journal from MDPI · 2024Article
- Presence ofPathogens (Basel, Switzerland) · 2023Article
- A Systematic Review of the Distribution of Tick-Borne Pathogens in Wild Animals and Their Ticks in the Mediterranean Rim between 2000 and 2021.Microorganisms · 2022Review
- Prevalence and Genotyping ofAnimals : an open access journal from MDPI · 2022Article
- Ecotyping ofAnimals : an open access journal from MDPI · 2021Article
- The absence of the drhm gene is not a marker for human-pathogenicity in European Anaplasma phagocytophilum strains.Parasites & vectors · 2020Article
- A review on the eco-epidemiology and clinical management of human granulocytic anaplasmosis and its agent in Europe.Parasites & vectors · 2019Review
- Presence of Roe Deer Affects the Occurrence ofInternational journal of environmental research and public health · 2019Article
- First detection and molecular identification of the zoonotic Anaplasma capra in deer in France.PloS one · 2019Article
- Diverse tick-borne microorganisms identified in free-living ungulates in Slovakia.Parasites & vectors · 2018Article
- Co-circulation of different A. phagocytophilum variants within cattle herds and possible reservoir role for cattle.Parasites & vectors · 2018Article
- Detection of Tick-Borne Pathogens in Lambs Undergoing Prophylactic Treatment Against Ticks on Two Swedish Farms.Frontiers in veterinary science · 2018Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAnaplasma phagocytophilum is a tick-transmitted Gram-negative obligate intracellular bacterium able to infect a wide variety of wild and domestic animals worldwide. Based on the genetic diversity observed with different molecular markers, several host-specific lineages have been identified. Roe deer is one of the most important reservoirs of this bacterium and hosts different genetic groups sometimes found on domestic animals. We therefore developed an ankA cluster-specific nested PCR (nPCR) to evaluate the prevalence of the three different ankA genetic groups described in roe deer (clusters II, III and IV) at three locations in France and the level of co-infections.
resultsThe specificity of the three nPCRs was assessed by partially sequencing 35 amplicons of ankA genes obtained from the different nested PCRs. All three genetic lineages were detected in roe deer from all three geographical locations. Of the infected deer population, 60.7% were co-infected by two or three different genetic variants. Co-infections varied from 42.9 to 70.6% of the infected population depending on the local infection prevalences (from 33.3 to 73.9%). All types of mixed infections occurred, suggesting the absence of a strict variant exclusion by another variant.
conclusionsMixed infections by two or three genetic variants of A. phagocytopilum are a common feature in roe deer. Genetic variants (cluster IV) also found in domestic ruminants (cattle and sheep) were present in all the roe deer populations analyzed, suggesting a shared epidemiological cycle.
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