Evidence map›Paper›PMID 28783176›Full record

ArticleOncogene2017

Short stretches of rare codons regulate translation of the transcription factor ZEB2 in cancer cells.

W R Wan Makhtar, G Browne, A Karountzos, C Stevens, Y Alghamdi, A R Bottrill, S Mistry, E Smith, M Bushel, J H Pringle and 2 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Oncogene, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed, 1 pooled it
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 1 synthesis or guideline pooled it, 26 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

W R Wan MakhtarDepartment of Cancer Studies, University of Leicester, Leicester, UK.
G BrowneDepartment of Cancer Studies, University of Leicester, Leicester, UK.
A KarountzosDepartment of Cancer Studies, University of Leicester, Leicester, UK.
C StevensDepartment of Cancer Studies, University of Leicester, Leicester, UK.
Y AlghamdiDepartment of Cancer Studies, University of Leicester, Leicester, UK.
A R BottrillProtein and Nucleic Acid Chemistry Laboratory (PNACL), University of Leicester, Leicester, UK.
S MistryProtein and Nucleic Acid Chemistry Laboratory (PNACL), University of Leicester, Leicester, UK.
E SmithMRC Toxicology Unit, Leicester, UK.
M BushelMRC Toxicology Unit, Leicester, UK.
J H PringleDepartment of Cancer Studies, University of Leicester, Leicester, UK.
A E SayanCancer Sciences Division, University of Southampton, Southampton, UK.
E TulchinskyDepartment of Cancer Studies, University of Leicester, Leicester, UK.
University of Leicester · GBMRC Toxicology Unit · GBUniversity of Southampton · GB

Funding

Cancer Research UK A10844Wellcome Trust
6 · The paper itself

Abstract

Two proteins comprising the ZEB family of zinc finger transcription factors, ZEB1 and ZEB2, execute EMT programs in embryonic development and cancer. By studying regulation of their expression, we describe a novel mechanism that limits ZEB2 protein synthesis. A protein motif located at the border of the SMAD-binding domain of ZEB2 protein induces ribosomal pausing and compromises protein synthesis. The function of this protein motif is dependent on stretches of rare codons, Leu(UUA)-Gly(GGU)-Val(GUA). Incorporation of these triplets in the homologous region of ZEB1 does not affect protein translation. Our data suggest that rare codons have a regulatory role only if they are present within appropriate protein structures. We speculate that pools of transfer RNA available for protein translation impact on the configuration of epithelial mesenchymal transition pathways in tumor cells.

Indexed as

Amino Acid MotifsCell Line, TumorCodonEpithelial-Mesenchymal TransitionGlycineHumansLeucineNeoplasmsProtein BiosynthesisRNA, Transfer, GlyRNA, Transfer, LeuRNA, Transfer, ValSignal TransductionValineZinc Finger E-box-Binding Homeobox 1Zinc Finger E-box Binding Homeobox 2CodonGlycineLeucineRNA, Transfer, GlyRNA, Transfer, LeuRNA, Transfer, ValValineZEB1 protein, humanZEB2 protein, humanZinc Finger E-box-Binding Homeobox 1Zinc Finger E-box Binding Homeobox 2

Identifiers

PMID28783176
PMCPMC5681250
OpenAlexW2742550718

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.