ArticleBiophysics reports2017
Particle segmentation algorithm for flexible single particle reconstruction.
Article in Biophysics reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Exceptionally potent human monoclonal antibodies are effective for prophylaxis and treatment of tetanus in mice.The Journal of clinical investigation · 2021Article
- Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution.Nature communications · 2019Article
- Mechanistic insights into the SNARE complex disassembly.Science advances · 2019Article
- In vivo assembly and large-scale purification of a GPCR - Gα fusion with Gβγ, and characterization of the active complex.PloS one · 2019Article
- Image processing for cryogenic transmission electron microscopy of symmetry-mismatched complexes.Bioscience reports · 2018Article
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Authors and funding
3 authors.
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Abstract
As single particle cryo-electron microscopy has evolved to a new era of atomic resolution, sample heterogeneity still imposes a major limit to the resolution of many macromolecular complexes, especially those with continuous conformational flexibility. Here, we describe a particle segmentation algorithm towards solving structures of molecules composed of several parts that are relatively flexible with each other. In this algorithm, the different parts of a target molecule are segmented from raw images according to their alignment information obtained from a preliminary 3D reconstruction and are subjected to single particle processing in an iterative manner. This algorithm was tested on both simulated and experimental data and showed improvement of 3D reconstruction resolution of each segmented part of the molecule than that of the entire molecule.
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