ArticleCurrent biology : CB2017
Identification of Chlamydomonas Central Core Centriolar Proteins Reveals a Role for Human WDR90 in Ciliogenesis.
Article in Current biology : CB, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 68 citations in OpenAlex.
- Meta-evolutionary exome analysis identifies novel type 2 diabetes mellitus genes in the UK Biobank and all of us.PLoS genetics · 2025Pooled it
- Systematic Reanalysis of Whole-Exome Sequencing in Genetically Unsolved Pediatric Primary Ciliary Dyskinesia.Pediatric pulmonology · 2026Article
- Prognostic, diagnostic and therapeutic value of the cancer tubulin code.Cancer metastasis reviews · 2026Review
- Tubulin glutamylation: a key regulator of flagella, cilia, centrosomes, and disease pathways.Journal of biomedical science · 2026Review
- Critical constituents and assembly principles of centriole biogenesis in human cells.Nature reviews. Molecular cell biology · 2026Review
- CFAP100 couples microtubule glutamylation to spindle pole integrity in keratinocytes to promote epidermal development.Nature communications · 2025Article
- A delta-tubulin/epsilon-tubulin/Ted protein complex is required for centriole architecture.eLife · 2025Article
- An interaction network of inner centriole proteins organised by POC1A-POC1B heterodimer crosslinks ensures centriolar integrity.Nature communications · 2024Article
- The Structure of Cilium Inner Junctions Revealed by Electron Cryo-tomography.bioRxiv : the preprint server for biology · 2024Article
- Fly Fam161 is an essential centriole and cilium transition zone protein with unique and diverse cell type-specific localizations.Open biology · 2024Article
- Poc1 bridges basal body inner junctions to promote triplet microtubule integrity and connections.The Journal of cell biology · 2024Article
- CCDC15 localizes to the centriole inner scaffold and controls centriole length and integrity.The Journal of cell biology · 2023Article
- Poc1 is a basal body inner junction protein that promotes triplet microtubule integrity and interconnections.bioRxiv : the preprint server for biology · 2023Article
- Prenatal benzene exposure in mice alters offspring hypothalamic development predisposing to metabolic disease in later life.Chemosphere · 2023Article
- Single-molecule imaging in the primary cilium.Methods in cell biology · 2023Article
- The central scaffold protein CEP350 coordinates centriole length, stability, and maturation.The Journal of cell biology · 2022Article
- TissUExM enables quantitative ultrastructural analysis in whole vertebrate embryos by expansion microscopy.Cell reports methods · 2022Article
- Article
- Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).Nature methods · 2022Article
- Tubulin posttranslational modifications modify the atypical spermatozoon centriole.microPublication biology · 2022Article
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Centrioles are evolutionarily conserved macromolecular structures that are fundamental to form cilia, flagella, and centrosomes. Centrioles are 9-fold symmetrical microtubule-based cylindrical barrels comprising three regions that can be clearly distinguished in the Chlamydomonas reinhardtii organelle: an ∼100-nm-long proximal region harboring a cartwheel; an ∼250-nm-long central core region containing a Y-shaped linker; and an ∼150-nm-long distal region ending at the transitional plate. Despite the discovery of many centriolar components, no protein has been localized specifically to the central core region in Chlamydomonas thus far. Here, combining relative quantitative mass spectrometry and super-resolution microscopy on purified Chlamydomonas centrioles, we identified POB15 and POC16 as two proteins of the central core region, the distribution of which correlates with that of tubulin glutamylation. We demonstrated that POB15 is an inner barrel protein within this region. Moreover, we developed an assay to uncover temporal relationships between centriolar proteins during organelle assembly and thus established that POB15 is recruited after the cartwheel protein CrSAS-6 and before tubulin glutamylation takes place. Furthermore, we discovered that two poc16 mutants exhibit flagellar defects, indicating that POC16 is important for flagellum biogenesis. In addition, we discovered that WDR90, the human homolog of POC16, localizes to a region of human centrioles that we propose is analogous to the central core of Chlamydomonas centrioles. Moreover, we demonstrate that WDR90 is required for ciliogenesis, echoing the findings in Chlamydomonas. Overall, our work provides novel insights into the identity and function of centriolar central core components.
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