ArticleThe ISME journal2017
Mixotrophy drives niche expansion of verrucomicrobial methanotrophs.
Article in The ISME journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers, 1 of them a synthesis that pooled it.
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Who cites it
62 citing papers in PubMed, 1 synthesis or guideline pooled it, 132 citations in OpenAlex.
- Leveraging genome-scale metabolic models to understand aerobic methanotrophs.The ISME journal · 2024Pooled it
- High-efficiency methane consumption by atmospheric methanotrophs in subsurface karst caves: The irrefutable methane sink.Science advances · 2026Article
- Simultaneous co-cultivation of the thermoacidophilic methanotroph,Engineering microbiology · 2025Article
- DSOF: A Rapid Method to Determine the Abundance of Microalgae and Methanotrophic Bacteria in Coculture Using a Combination of Differential Sedimentation, Optical Density, and Fluorescence.Bioengineering (Basel, Switzerland) · 2025Article
- Hydrogen Oxidation Benefits Alphaproteobacterial Methanotrophs Under Severe Methane Limitation.Environmental microbiology · 2025Article
- Mosses as extraordinary reservoir of microbial diversity: a comparative analysing of co-occurring 'plant-moss twins' in natural alpine ecosystem.Environmental microbiome · 2025Article
- Genomic characterisation of novel extremophile lineages from the thalassohaline lake Dziani Dzaha expands the metabolic repertoire of the PVC superphylum.Environmental microbiome · 2025Article
- Regulators of aerobic and anaerobic methane oxidation in two pristine temperate peatland types.FEMS microbiology ecology · 2024Article
- Ethylene and epoxyethane metabolism in methanotrophic bacteria: comparative genomics and physiological studies usingMicrobial genomics · 2024Article
- Isolation of a facultative methanotroph Methylocystis iwaonis SD4 from rice rhizosphere and establishment of rapid genetic tools for it.Biotechnology letters · 2024Article
- Trace gas oxidation sustains energy needs of a thermophilic archaeon at suboptimal temperatures.Nature communications · 2024Article
- Genome-scale flux balance analysis reveals redox trade-offs in the metabolism of the thermoacidophileFrontiers in systems biology · 2024Article
- Microbial activity contributes to spatial heterogeneity of wetland methane fluxes.Microbiology spectrum · 2023Article
- Characterisation of 'Candidatus Methylobacter titanis' sp. nov., a putative novel species of Methylobacter clade 2 and their distribution in sediments of freshwater lakes in maritime Antarctica.Antonie van Leeuwenhoek · 2023Article
- Simultaneous sulfide and methane oxidation by an extremophile.Nature communications · 2023Article
- Mixotrophy broadens the ecological niche range of the iron oxidizer Sideroxydans sp. CL21 isolated from an iron-rich peatland.FEMS microbiology ecology · 2023Article
- Thermophilic methane oxidation is widespread in Aotearoa-New Zealand geothermal fields.Frontiers in microbiology · 2023Article
- Microbial oxidation of atmospheric trace gases.Nature reviews. Microbiology · 2022Review
- Diversity and Composition of Methanotroph Communities in Caves.Microbiology spectrum · 2022Article
- Sulfur and methane oxidation by a single microorganism.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 6 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aerobic methanotrophic bacteria have evolved a specialist lifestyle dependent on consumption of methane and other short-chain carbon compounds. However, their apparent substrate specialism runs contrary to the high relative abundance of these microorganisms in dynamic environments, where the availability of methane and oxygen fluctuates. In this work, we provide in situ and ex situ evidence that verrucomicrobial methanotrophs are mixotrophs. Verrucomicrobia-dominated soil communities from an acidic geothermal field in Rotokawa, New Zealand rapidly oxidised methane and hydrogen simultaneously. We isolated and characterised a verrucomicrobial strain from these soils, Methylacidiphilum sp. RTK17.1, and showed that it constitutively oxidises molecular hydrogen. Genomic analysis confirmed that this strain encoded two [NiFe]-hydrogenases (group 1d and 3b), and biochemical assays revealed that it used hydrogen as an electron donor for aerobic respiration and carbon fixation. While the strain could grow heterotrophically on methane or autotrophically on hydrogen, it grew optimally by combining these metabolic strategies. Hydrogen oxidation was particularly important for adaptation to methane and oxygen limitation. Complementary to recent findings of hydrogenotrophic growth by Methylacidiphilum fumariolicum SolV, our findings illustrate that verrucomicrobial methanotrophs have evolved to simultaneously utilise hydrogen and methane from geothermal sources to meet energy and carbon demands where nutrient flux is dynamic. This mixotrophic lifestyle is likely to have facilitated expansion of the niche space occupied by these microorganisms, allowing them to become dominant in geothermally influenced surface soils. Genes encoding putative oxygen-tolerant uptake [NiFe]-hydrogenases were identified in all publicly available methanotroph genomes, suggesting hydrogen oxidation is a general metabolic strategy in this guild.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.