Evidence map›Paper›PMID 28777088›Full record

ArticleActa crystallographica. Section F, Structural biology communications2017

Production, biophysical characterization and crystallization of Pseudomonas putida GraA and its complexes with GraT and the graTA operator.

Ariel Talavera, Hedvig Tamman, Andres Ainelo, San Hadǽi, Abel Garcia-Pino, Rita Hõrak, Albert Konijnenberg, Remy Loris

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 3 countries.

Ariel TalaveraStructural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium.
Hedvig TammanInstitute of Molecular and Cell Biology, University of Tartu, 51010 Tartu, Estonia.
Andres AineloInstitute of Molecular and Cell Biology, University of Tartu, 51010 Tartu, Estonia.
San HadǽiStructural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium.
Abel Garcia-PinoInstitut de Biologie et de Médecine Moléculaires, Université Libre de Bruxelles, 6041 Gosselies, Belgium.
Rita HõrakInstitute of Molecular and Cell Biology, University of Tartu, 51010 Tartu, Estonia.
Albert KonijnenbergStructural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium.
Remy LorisStructural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium.
University of Tartu · EEVrije Universiteit Brussel · BEUniversité Libre de Bruxelles · BEVIB-VUB Center for Structural Biology · BE

Funding

Estonian Research Council IUT20-19Estonian Research Council PUT1351Fonds Wetenschappelijk Onderzoek G.0135.15NFonds Wetenschappelijk Onderzoek G0C1213NThe Hercules Foundation UABR/11/012Vrije Universiteit Brussel OZR2232Vrije Universiteit Brussel SPR13
6 · The paper itself

Abstract

The graTA operon from Pseudomonas putida encodes a toxin-antitoxin module with an unusually moderate toxin. Here, the production, SAXS analysis and crystallization of the antitoxin GraA, the GraTA complex and the complex of GraA with a 33 bp operator fragment are reported. GraA forms a homodimer in solution and crystallizes in space group P2

Indexed as

Gene Expression Regulation, BacterialAmino Acid SequenceAntitoxinsBacterial ProteinsBacterial ToxinsCloning, MolecularCrystallizationCrystallography, X-RayEscherichia coliGene ExpressionGenetic VectorsOperonProtein BindingProtein MultimerizationPseudomonas putidaRecombinant Fusion ProteinsAntitoxinsBacterial ProteinsBacterial ToxinsRecombinant Fusion ProteinsGraAGraTmacromolecular complexpersistenceprotein–DNA complexPseudomonas putidaribosome biogenesistoxin–antitoxin module

Identifiers

PMID28777088
PMCPMC5544002
OpenAlexW2740431636

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.