ArticlePlant molecular biology2017
Bypassing miRNA-mediated gene regulation under drought stress: alternative splicing affects CSD1 gene expression.
Article in Plant molecular biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 51 citations in OpenAlex.
- Genomic basis underlying alternative transcripts-mediated drought tolerance in maize.Genome biology · 2026Article
- Integrating Drought Stress Signaling and Smart Breeding for Climate-Resilient Crops: Regulatory Mechanisms and Genetic Strategies.Plants (Basel, Switzerland) · 2025Review
- Non-coding RNAs in plant stress responses: molecular insights and agricultural applications.Plant biotechnology journal · 2025Review
- Maize miRNAs and their putative target genes involved in chilling stress response in 5-day old seedlings.BMC genomics · 2024Article
- Regulation of endogenous hormone and miRNA in leaves of alfalfa (Medicago sativa L.) seedlings under drought stress by endogenous nitric oxide.BMC genomics · 2024Article
- Function identification of miR159a, a positive regulator during poplar resistance to drought stress.Horticulture research · 2023Article
- MicroRNA398: A Master Regulator of Plant Development and Stress Responses.International journal of molecular sciences · 2022Review
- Genome-Wide Analysis and Expression Profile of Superoxide Dismutase (SOD) Gene Family in Rapeseed (Antioxidants (Basel, Switzerland) · 2021Article
- The role of RNA-binding protein, microRNA and alternative splicing in seed germination: a field need to be discovered.BMC plant biology · 2021Review
- Catalase (CAT) Gene Family in Rapeseed (International journal of molecular sciences · 2021Article
- Review
- Integration of sRNA, degradome, transcriptome analysis and functional investigation reveals gma-miR398c negatively regulates drought tolerance via GmCSDs and GmCCS in transgenic Arabidopsis and soybean.BMC plant biology · 2020Article
- Sugar Signaling and Post-transcriptional Regulation in Plants: An Overlooked or an Emerging Topic?Frontiers in plant science · 2020Review
- Functional characterization of Gh_A08G1120 (GH3.5) gene reveal their significant role in enhancing drought and salt stress tolerance in cotton.BMC genetics · 2019Article
- Comparative transcriptome and translatome analysis in contrasting rice genotypes reveals differential mRNA translation in salt-tolerant Pokkali under salt stress.BMC genomics · 2018Article
- Chilling and Drought Stresses in Crop Plants: Implications, Cross Talk, and Potential Management Opportunities.Frontiers in plant science · 2018Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageThe binding site for miR398 in an isoform of Cu/Zn superoxide dismutase (CSD1) is eliminated by alternative splicing to bypass miR398-mediated gene down-regulation under drought stress. MicroRNA (miRNA) binding sites (MBSs) are frequently interrupted by introns and therefore require proper splicing to generate functional MBSs in target transcripts. MBSs can also be excluded during splicing of pre-messenger RNA, leading to different regulation among isoforms. Previous studies have shown that levels of Cu/Zn superoxide dismutase (CSD) are down-regulated by miR398. In this study, sequences and transcript levels of peanut CSD1 isoforms (AhCSD1-1, AhCSD1-2.1, and AhCSD1-2.2) were analyzed under the drought stress. Results demonstrated that a miR398 binding site is eliminated in AhCSD1-2.2 as a consequence of alternative splicing, which bypasses miRNA-mediated down-regulation under drought stress. This alternative isoform was not only identified in peanut but also in soybean and Arabidopsis. In addition, transgenic Arabidopsis plants expressing AhCSD1 were more tolerant to osmotic stress. We hypothesize that the level of AhCSD1 is increased to allow diverse plant responses to overcome environmental challenges even in the presence of increased miR398 levels. These findings suggest that studies on the role of alternatively spliced MBSs affecting transcript levels are important for understanding plant stress responses.
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