ArticleScience advances2017
Three-dimensional positioning and structure of chromosomes in a human prophase nucleus.
Article in Science advances, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 52 citations in OpenAlex.
- Uncovering mitotic ultrastructure in the native hair follicle using volume electron microscopy.Journal of cell science · 2026Article
- One step 4× and 12× 3D-ExM enables robust super-resolution microscopy of nanoscale cellular structures.The Journal of cell biology · 2025Article
- One step 4x and 12x 3D-ExM: robust super-resolution microscopy in cell biology.bioRxiv : the preprint server for biology · 2024Article
- Depletion of lamins B1 and B2 promotes chromatin mobility and induces differential gene expression by a mesoscale-motion-dependent mechanism.Genome biology · 2024Article
- Sample preparation and data collection for serial block face scanning electron microscopy of mammalian cell monolayers.PloS one · 2024Article
- Micro-Topographies Induce Epigenetic Reprogramming and Quiescence in Human Mesenchymal Stem Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Stochastic chromatin packing of 3D mitotic chromosomes revealed by coherent X-rays.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Gravitational Force-Induced 3D Chromosomal Conformational Changes Are Associated with Rapid Transcriptional Response in Human T Cells.International journal of molecular sciences · 2021Article
- Ultra-Structural Imaging Provides 3D Organization of 46 Chromosomes of a Human Lymphocyte Prophase Nucleus.International journal of molecular sciences · 2021Article
- Contribution of advanced fluorescence nano microscopy towards revealing mitotic chromosome structure.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Review
- Chromosome inner structure investigation by electron tomography and electron diffraction in a transmission electron microscope.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Review
- X-ray Ptychography Imaging of Human Chromosomes After Low-dose Irradiation.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Article
- Cryo-nanoscale chromosome imaging-future prospects.Biophysical reviews · 2020Review
- Mitotic chromosome organization: General rules meet species-specific variability.Computational and structural biotechnology journal · 2020Review
- Use of 3D imaging for providing insights into high-order structure of mitotic chromosomes.Chromosoma · 2019Review
- Investigation of Three-Dimensional Structure and Pigment Surrounding Environment of a TiO₂ Containing Waterborne Paint.Materials (Basel, Switzerland) · 2019Article
- Interchromosomal interactions: A genomic love story of kissing chromosomes.The Journal of cell biology · 2019Review
- Three-dimensional imaging and analysis of the internal structure of SAPO-34 zeolite crystals.RSC advances · 2018Article
- Capsid-CPSF6 Interaction Licenses Nuclear HIV-1 Trafficking to Sites of Viral DNA Integration.Cell host & microbe · 2018Article
- Investigation of Three-Dimensional Microstructure of Tricalcium Silicate (C₃S) by Electron Microscopy.Materials (Basel, Switzerland) · 2018Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human genetic material is packaged into 46 chromosomes. The structure of chromosomes is known at the lowest level, where the DNA chain is wrapped around a core of eight histone proteins to form nucleosomes. Around a million of these nucleosomes, each about 11 nm in diameter and 6 nm in thickness, are wrapped up into the complex organelle of the chromosome, whose structure is mostly known at the level of visible light microscopy to form a characteristic cross shape in metaphase. However, the higher-order structure of human chromosomes, between a few tens and hundreds of nanometers, has not been well understood. We show a three-dimensional (3D) image of a human prophase nucleus obtained by serial block-face scanning electron microscopy, with 36 of the complete set of 46 chromosomes captured within it. The acquired image allows us to extract quantitative 3D structural information about the nucleus and the preserved, intact individual chromosomes within it, including their positioning and full spatial morphology at a resolution of around 50 nm in three dimensions. The chromosome positions were found, at least partially, to follow the pattern of chromosome territories previously observed only in interphase. The 3D conformation shows parallel, planar alignment of the chromatids, whose occupied volumes are almost fully accounted for by the DNA and known chromosomal proteins. We also propose a potential new method of identifying human chromosomes in three dimensions, on the basis of the measurements of their 3D morphology.
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