Evidence map›Paper›PMID 28775968›Full record

ArticleJournal of molecular biochemistry2017

The Role of S-Palmitoylation of the Human Glucocorticoid Receptor (hGR) in Mediating the Nongenomic Glucocorticoid Actions.

Nicolas C Nicolaides, Tomoshige Kino, Michael L Roberts, Eleni Katsantoni, Amalia Sertedaki, Paraskevi Moutsatsou, Anna-Maria G Psarra, George P Chrousos, Evangelia Charmandari

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Journal of molecular biochemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.3field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 18 citations in OpenAlex.

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  12. Non-genomic Effects of Glucocorticoids: An Updated View.Trends in pharmacological sciences · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

Nicolas C NicolaidesDivision of Endocrinology and Metabolism, Center of Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
Tomoshige KinoProgram in Reproductive and Adult Endocrinology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland.
Michael L RobertsDivision of Endocrinology and Metabolism, Center of Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
Eleni KatsantoniDivision of Hematology-Oncology, Basic Research Center, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
Amalia SertedakiDivision of Endocrinology, Metabolism and Diabetes, First Department of Pediatrics, National and Kapodistrian University of Athens Medical School, "Aghia Sophia" Children's Hospital, Athens, Greece.
Paraskevi MoutsatsouDepartment of Clinical Biochemistry, University of Athens Medical School, "Attiko" Hospital, Athens, 12462, Greece.
Anna-Maria G PsarraDepartment of Biochemistry and Biotechnology, University of Thessaly, Larissa, 41221, Greece.
George P ChrousosDivision of Endocrinology and Metabolism, Center of Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
Evangelia CharmandariDivision of Endocrinology and Metabolism, Center of Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
Biomedical Research Foundation of the Academy of Athens · GRNational and Kapodistrian University of Athens · GRNational Institutes of Health · USUniversity of Thessaly · GR

Funding

Vpr A Viral Coactivator Of Nuclear Receptors--MechanismsZ01HD008732 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI KINO, TOMOSHIGE · 2001 to 2008
$511k
Intramural NIH HHS Z01 HD008732
6 · The paper itself

Abstract

backgroundMany rapid nongenomic glucocorticoid actions are mediated by membrane-bound glucocorticoid receptors (GRs). S-palmitoylation is a lipid post-translational modification that mediates the membrane localization of some steroid receptors. A highly homologous amino acid sequence (663YLCM KTLLL671) is present in the ligand-binding domain of hGRα, suggesting that hGRα might also undergo S-palmitoylation.

aimTo investigate the role of the motif 663YLCMKTLLL671 in membrane localization of the hGRα and in mediating rapid nongenomic glucocorticoid signaling. METHODS AND

resultsWe showed that the mutant receptors hGRαY663A, hGRαC665A and hGRαLL670/671AA, and the addition of the palmitoylation inhibitor 2-bromopalmitate did not prevent membrane localization of hGRα and co-localization with caveolin-1, and did not influence the biphasic activation of mitogen-activated protein kinase (MAPK) signaling pathway in the early time points. Finally, the hGRα was not shown to undergo S-palmitoylation.

conclusionsThe motif 663YLCMKTLLL671 does not play a role in membrane localization of hGRα and does not mediate the nongenomic glucocorticoid actions.

Identifiers

PMID28775968
PMCPMC5538142
OpenAlexW2611141167

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.