Evidence map›Paper›PMID 28775823›Full record

ArticleIranian journal of microbiology2017

Expression and purification of recombinant HTLV-I/-II linear epitopes antigen and its application for screening of suspected patients.

Roxana Faramarzi, Samaneh Dolatabadi

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Iranian journal of microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Roxana FaramarziDepartment of Microbiology, Neyshabur Branch, Islamic Azad University, Neyshabur, Khorasan Razavi, Iran.
Samaneh DolatabadiDepartment of Microbiology, Neyshabur Branch, Islamic Azad University, Neyshabur, Khorasan Razavi, Iran.
Islamic Azad University of Nishapur · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND AND

objectivesThe linear epitopes of gp46-I, gp46-II, gp21 and p19 are used in diagnosis of HTLV-I/-II infections. The aims of this study was to obtain high-level expression and purification of recombinant antigen (RA) containing these epitopes. Large-scale preparation of such antigen probably worths for diagnostic purpose. MATERIALS AND

methodsThe synthetic DNA encoding RA was synthesized and over-expressed as soluble in

resultsSDS-PAGE revealed that the purified protein was more than 90% pure. The final yield was approximately 25 mg per liter of culture medium. ELISA results showed that RA could specifically bind to anti-HTLV-I/-II antibodies in infected sera.

conclusionRA could be a candidate for HTLV-I/-II screening and the strategy presented in this study could be used for easy production of this diagnostic protein.

Indexed as

Chimeric antigenHTLVProtein expressionPurification

Identifiers

PMID28775823
PMCPMC5534004
OpenAlexW2614556575

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.