Evidence map›Paper›PMID 28775822›Full record

ArticleIranian journal of microbiology2017

An evaluation study on phenotypical methods and real-time PCR for detection of

Shirin Sadr, Davood Darban-Sarokhalil, Gholam Reza Irajian, Abbas Ali Imani Fooladi, Jaleh Moradi, Mohammad Mehdi Feizabadi

Abstract read
In one paragraph

Article in Iranian journal of microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Rapid detection ofThe Pan African medical journal · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Shirin SadrDepartment of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Davood Darban-SarokhalilDepartment of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Gholam Reza IrajianDepartment of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Abbas Ali Imani FooladiApplied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Jaleh MoradiDepartment of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Mohammad Mehdi FeizabadiDepartment of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND AND

objectivesFor further confirmation of the previous in-house real-time PCR and CYP 141 target as a rapid and cheap diagnostic technique and a new target for direct detection of MATERIALS AND

methodsThirty two sputa (including 15 smear positive and 17 smear negative) and 53 Sputa (29 smear and culture positive and 24 smear and culture negative specimens) were collected from tuberculosis suspected patients from health center No. 1 and 2 respectively. A Taqman probe was used for direct detection of

resultsBecause of the results, data of health center No. 1 was not reliable. The average number of bacteria that was detected in health center No. 2 by real time PCR was 1.2E+003-7.3+004; 1.4E+004-1.29+005 and 8.27E+005-1.07+006 for one to three plus smear result groups, respectively. The overall sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of real-time PCR were 96.5% (28/29), 95.8% (23/24), (96.6%) and (96%), respectively.

conclusionCompared with the results of previous studies and being a good correlation between real-time PCR and phenotypic methods, emphasize that CYP141 is a good target for quantification of

Indexed as

Cytochrome P450 cyp141Mycobacterium tuberculosisReal-time PCR

Identifiers

PMID28775822
PMCPMC5534003

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.