ArticleMobile DNA2017
Targeted identification of TE insertions in a
Article in Mobile DNA, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
6 citing papers in PubMed, 10 citations in OpenAlex.
- Adaptive P-element Insertions in a Long Non-Coding RNA are Potential Emerging piRNA Clusters.Genome biology and evolution · 2026Article
- Uracil walking primer PCR: An accurate and efficient genome-walking tool.Journal, genetic engineering & biotechnology · 2025Article
- P-element invasion fuels molecular adaptation in laboratory populations of Drosophila melanogaster.Evolution; international journal of organic evolution · 2023Article
- Rapid evolution of piRNA-mediated silencing of an invading transposable element was driven by abundant de novo mutations.Genome research · 2020Article
- Population-specific dynamics and selection patterns of transposable element insertions in European natural populations.Molecular ecology · 2019Article
- The Evolution of Small-RNA-Mediated Silencing of an Invading Transposable Element.Genome biology and evolution · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTransposable elements (TEs) are major components of eukaryotic genomes and drivers of genome evolution, producing intraspecific polymorphism and interspecific differences through mobilization and non-homologous recombination. TE insertion sites are often highly variable within species, creating a need for targeted genome re-sequencing (TGS) methods to identify TE insertion sites.
methodsWe present a hemi-specific PCR approach for TGS of
resultsBy comparing our results to TE annotations based on whole genome re-sequencing (WGS) data for the same
conclusionsTGS by hemi-specific PCR is a powerful approach for identifying TE insertions of particular TE families in species with a high-quality reference genome, at greatly reduced cost as compared to WGS. It may therefore be ideal for population genomic studies of particular TE families. Additionally, TGS and WGS can be used as complementary approaches, with TGS annotations identifying more annotated insertions with greater precision for a target TE family, and WGS data allowing for estimates of TE insertion frequencies, and a broader picture of the location of non-target TEs across the genome.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.