ArticleIranian journal of veterinary research2017
LAMP assay for rapid diagnosis of cow DNA in goat milk and meat samples.
Article in Iranian journal of veterinary research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 13 citations in OpenAlex.
- Development of point-of-need colourimetric, isothermal diagnostic assays for specific detection of Bacillus subtilis using shikimate dehydrogenase gene.Folia microbiologica · 2025Article
- Development and Application of a Visual Duck Meat Detection Strategy for Molecular Diagnosis of Duck-Derived Components.Foods (Basel, Switzerland) · 2022Article
- Recombinase Polymerase Amplification Based Multiplex Lateral Flow Dipstick for Fast Identification of Duck Ingredient in Adulterated Beef.Animals : an open access journal from MDPI · 2020Article
- Development of a rapid, one-step-visual method to detectIranian journal of veterinary research · 2020Article
- Specific and rapid identification of theBioscience reports · 2019Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Animal species detection is one of the crucial steps for consumer's food analysis. In the present study we developed an in-house built loop-mediated isothermal amplification (LAMP) assay for rapid detection of adulterated cow DNA in goat milk/meat samples. The cow milk/tissue DNA in goat milk/meat samples were identified in the developed LAMP assay by either naked eye visualizing with SYBR Green I dyes or by detecting the typical ladder pattern on gel electrophoresis. This test can detect up to minimum 5% level of cow components admixed in goat milk/meat samples and can be completed within 1 h 40 min starting from DNA extraction from milk/meat samples and can be performed in a water bath. Developed LAMP methodology is simple; rapid and sensitive techniques that can detect adulterant like cow components in goat milk/meat are more accurate than other existing DNA based technologies.
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Identifiers
28775755PMC5534258W2716743154What OpenQuestion holds
Registered trials
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