ArticleScientific reports2017
Development of SimCells as a novel chassis for functional biosensors.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Advanced biosensing strategies for high-risk foodborne pathogens: a comprehensive review ofFood chemistry. Molecular sciences · 2026Review
- Reprogrammed SimCells for antimicrobial therapy.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- A Safe and Versatile Minicell Platform Derived fromJournal of microbiology and biotechnology · 2025Article
- Bacterial Minicell-Based Biohybrid Sub-micron Swimmers for Targeted Cargo Delivery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Motile and Chemotactic Minicells and Minicell-Driven Biohybrids Engineered for Active Cargo Delivery.ACS applied materials & interfaces · 2025Article
- Leveraging EngineeredACS synthetic biology · 2025Article
- Research advancements in nanoparticles and cell-based drug delivery systems for the targeted killing of cancer cells.Oncology research · 2025Review
- Fabrication of cyborg bacterial cells as living cell-material hybrids using intracellular hydrogelation.Nature protocols · 2024Review
- Bacterial therapies at the interface of synthetic biology and nanomedicine.Nature reviews bioengineering · 2024Article
- Reprogramming Synthetic Cells for Targeted Cancer Therapy.ACS synthetic biology · 2022Article
- Deterministic Lateral Displacement Microfluidic Chip for Minicell Purification.Micromachines · 2022Article
- Microbial Biocontainment Systems for Clinical, Agricultural, and Industrial Applications.Frontiers in bioengineering and biotechnology · 2022Review
- Production and Characterization of Motile and Chemotactic Bacterial Minicells.ACS synthetic biology · 2021Article
- Transient non-specific DNA binding dominates the target search of bacterial DNA-binding proteins.Molecular cell · 2021Article
- Chromosome-free bacterial cells are safe and programmable platforms for synthetic biology.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Comprehensive Profiling of Diverse Genetic Reporters with Application to Whole-Cell and Cell-Free Biosensors.Analytical chemistry · 2019Article
- Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals.Nature chemical biology · 2019Article
- Development of Aspirin-Inducible Biosensors inApplied and environmental microbiology · 2019Article
- The Synthetic Microbiology Caucus: from abstract ideas to turning microbes into cellular machines and back.Microbial biotechnology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
This work serves as a proof-of-concept for bacterially derived SimCells (Simple Cells), which contain the cell machinery from bacteria and designed DNA (or potentially a simplified genome) to instruct the cell to carry out novel, specific tasks. SimCells represent a reprogrammable chassis without a native chromosome, which can host designed DNA to perform defined functions. In this paper, the use of Escherichia coli MC1000 ∆minD minicells as a non-reproducing chassis for SimCells was explored, as demonstrated by their ability to act as sensitive biosensors for small molecules. Highly purified minicells derived from E. coli strains containing gene circuits for biosensing were able to transduce the input signals from several small molecules (glucarate, acrylate and arabinose) into the production of green fluorescent protein (GFP). A mathematical model was developed to fit the experimental data for induction of gene expression in SimCells. The intracellular ATP level was shown to be important for SimCell function. A purification and storage protocol was developed to prepare SimCells which could retain their functions for an extended period of time. This study demonstrates that SimCells are able to perform as 'smart bioparticles' controlled by designed gene circuits.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.