ArticleScientific reports2017
Occurrence and expression of novel methyl-coenzyme M reductase gene (mcrA) variants in hot spring sediments.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Metagenomic mining reveals extensive novelty, enhanced biodegradation potential, and untapped biosynthetic capacity in Chinese oilfield microbiomes.Applied and environmental microbiology · 2026Article
- Article
- Thermophilic Hadarchaeota grow on long-chain alkanes in syntrophy with methanogens.Nature communications · 2024Article
- Article
- Article
- A PCR-Based Survey of Methane-Cycling Archaea in Methane-Soaked Subsurface Sediments of Guaymas Basin, Gulf of California.Microorganisms · 2023Article
- Mcr-dependent methanogenesis in Archaeoglobaceae enriched from a terrestrial hot spring.The ISME journal · 2023Article
- Evidence for nontraditionalScience advances · 2023Article
- Diversity and function of methyl-coenzyme M reductase-encoding archaea in Yellowstone hot springs revealed by metagenomics and mesocosm experiments.ISME communications · 2023Article
- Foliage of Tropical Trees and Shrubs and Their Secondary Metabolites Modify In Vitro Ruminal Fermentation, Methane and Gas Production without a Tight Correlation with the Microbiota.Animals : an open access journal from MDPI · 2022Article
- Subsurface hydrocarbon degradation strategies in low- and high-sulfate coal seam communities identified with activity-based metagenomics.NPJ biofilms and microbiomes · 2022Article
- Hot in Cold: Microbial Life in the Hottest Springs in Permafrost.Microorganisms · 2020Article
- Diversity, ecology and evolution of Archaea.Nature microbiology · 2020Review
- Review
- Comparative evaluation of three archaeal primer pairs for exploring archaeal communities in deep-sea sediments and permafrost soils.Extremophiles : life under extreme conditions · 2019Article
- Microbial Community Structure and Methane Cycling Potential along a Thermokarst Pond-Peatland Continuum.Microorganisms · 2019Article
- Trophic strategy of diverse methanogens across a river-to-sea gradient.Journal of microbiology (Seoul, Korea) · 2019Article
- Divergent methyl-coenzyme M reductase genes in a deep-subseafloor Archaeoglobi.The ISME journal · 2019Article
- An evolving view of methane metabolism in the Archaea.Nature reviews. Microbiology · 2019Review
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent discoveries have shown that the marker gene for anaerobic methane cycling (mcrA) is more widespread in the Archaea than previously thought. However, it remains unclear whether novel mcrA genes associated with the Bathyarchaeota and Verstraetearchaeota are distributed across diverse environments. We examined two geochemically divergent but putatively methanogenic regions of Yellowstone National Park to investigate whether deeply-rooted archaea possess and express novel mcrA genes in situ. Small-subunit (SSU) rRNA gene analyses indicated that Bathyarchaeota were predominant in seven of ten sediment layers, while the Verstraetearchaeota and Euryarchaeota occurred in lower relative abundance. Targeted amplification of novel mcrA genes suggested that diverse taxa contribute to alkane cycling in geothermal environments. Two deeply-branching mcrA clades related to Bathyarchaeota were identified, while highly abundant verstraetearchaeotal mcrA sequences were also recovered. In addition, detection of SSU rRNA and mcrA transcripts from one hot spring suggested that predominant Bathyarchaeota were also active, and that methane cycling genes are expressed by the Euryarchaeota, Verstraetearchaeota, and an unknown lineage basal to the Bathyarchaeota. These findings greatly expand the diversity of the key marker gene for anaerobic alkane cycling and outline the need for greater understanding of the functional capacity and phylogenetic affiliation of novel mcrA variants.
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