Evidence map›Paper›PMID 28775309›Full record

ArticleScientific reports2017

Rapid de novo assembly of the European eel genome from nanopore sequencing reads.

Hans J Jansen, Michael Liem, Susanne A Jong-Raadsen, Sylvie Dufour, Finn-Arne Weltzien, William Swinkels, Alex Koelewijn, Arjan P Palstra, Bernd Pelster, Herman P Spaink and 3 more

Erratum issuedOpen access · goldAbstract read
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 63 papers.

0numbers the graph read from it
0cells of the map it votes in
63citing papers in PubMed
9.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

63 citing papers in PubMed, 123 citations in OpenAlex.

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  17. Estimation of Genome Size in the Endemic SpeciesPlants (Basel, Switzerland) · 2021
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3 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors at 6 institutions in 4 countries.

Hans J JansenZF-screens B.V., Leiden, The Netherlands.
Michael LiemInstitute of Biology, Leiden University, Leiden, The Netherlands.
Susanne A Jong-RaadsenZF-screens B.V., Leiden, The Netherlands.
Sylvie DufourMuséum National d'Histoire Naturelle, Sorbonne Universités, Research Unit BOREA, Biology of Aquatic Organisms and Ecosystems, CNRS, IRD, UCN, UA, Paris, France.
Finn-Arne WeltzienNorwegian University of Life Sciences, Faculty of Veterinary Medicine, Department of Basic Science and Aquatic Medicine, Oslo, Norway.ORCID http://orcid.org/0000-0002-5111-1558
William SwinkelsDUPAN Foundation, Wageningen, The Netherlands.
Alex KoelewijnDUPAN Foundation, Wageningen, The Netherlands.
Arjan P PalstraAnimal Breeding and Genomics Centre, Wageningen Livestock Research, Wageningen University & Research, Wageningen, The Netherlands.
Bernd PelsterInstitute of Zoology and Center for Molecular Biosciences, University of Innsbruck, Innsbruck, Austria.
Herman P SpainkInstitute of Biology, Leiden University, Leiden, The Netherlands.
Guido E van den ThillartZF-screens B.V., Leiden, The Netherlands.
Ron P DirksZF-screens B.V., Leiden, The Netherlands.
Christiaan V HenkelInstitute of Biology, Leiden University, Leiden, The Netherlands. c.v.henkel@biology.leidenuniv.nl.ORCID http://orcid.org/0000-0001-9838-8215
ZF Screens (Netherlands) · NLLeiden University · NLCentre National de la Recherche Scientifique · FRNorwegian University of Life Sciences · NOUniversität Innsbruck · ATWageningen University & Research · NL

Funding

Austrian Science Fund FWF I 2984Austrian Science Fund FWF P 26363
6 · The paper itself

Abstract

We have sequenced the genome of the endangered European eel using the MinION by Oxford Nanopore, and assembled these data using a novel algorithm specifically designed for large eukaryotic genomes. For this 860 Mbp genome, the entire computational process takes two days on a single CPU. The resulting genome assembly significantly improves on a previous draft based on short reads only, both in terms of contiguity (N50 1.2 Mbp) and structural quality. This combination of affordable nanopore sequencing and light weight assembly promises to make high-quality genomic resources accessible for many non-model plants and animals.

Indexed as

GenomeGenomicsHigh-Throughput Nucleotide SequencingAnimalsComputational BiologyEelsGenome SizeNanoporesSequence Analysis, DNA

Identifiers

PMID28775309
PMCPMC5543108
OpenAlexW2951397007

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.