ArticleScientific reports2017
Ectoine can enhance structural changes in DNA in vitro.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 44 citations in OpenAlex.
- The Potential of Ectoine as a Brain Anti-Aging Agent in Rat Model of D-Galactose-Accelerated Aging May Be Mediated Through Crosstalk Between Redox/Mitochondrial Homeostasis/Autophagic/Apoptotic Pathways.Molecular neurobiology · 2025Article
- Fullerenol CInternational journal of nanomedicine · 2024Article
- Ectoine Globally Hypomethylates DNA in Skin Cells and Suppresses Cancer Proliferation.Marine drugs · 2023Article
- Metabolic engineering of Halomonas campaniensis strain XH26 to remove competing pathways to enhance ectoine production.Scientific reports · 2023Article
- Genomic Investigation of DesertMicroorganisms · 2022Article
- Bioactivity profiling of the extremolyte ectoine as a promising protectant and its heterologous production.3 Biotech · 2022Review
- DNA Stability in Biodosimetry, Pharmacy and DNA Based Data-Storage: Optimal Storage and Handling Conditions.Chembiochem : a European journal of chemical biology · 2022Article
- Visualization of DNA Damage and Protection by Atomic Force Microscopy in Liquid.International journal of molecular sciences · 2022Article
- Raman Spectroscopic Signature of Ectoine Conformations in Bulk Solution and Crystalline State.Chemphyschem : a European journal of chemical physics and physical chemistry · 2020Article
- The architecture of the diaminobutyrate acetyltransferase active site provides mechanistic insight into the biosynthesis of the chemical chaperone ectoine.The Journal of biological chemistry · 2020Article
- The Skin-Whitening Effects of Ectoine via the Suppression of α-MSH-Stimulated Melanogenesis and the Activation of Antioxidant Nrf2 Pathways in UVA-Irradiated Keratinocytes.Antioxidants (Basel, Switzerland) · 2020Article
- The promotion and suppression of DNA charge neutralization by the cosolute ectoine.RSC advances · 2019Article
- The inflammation-reducing compatible solute ectoine does not impair the cytotoxic effect of ionizing radiation on head and neck cancer cells.Scientific reports · 2019Article
- Exploiting Substrate Promiscuity of Ectoine Hydroxylase for Regio- and Stereoselective Modification of Homoectoine.Frontiers in microbiology · 2019Article
- Review
- Ectoine protects DNA from damage by ionizing radiation.Scientific reports · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Strand breaks and conformational changes of DNA have consequences for the physiological role of DNA. The natural protecting molecule ectoine is beneficial to entire bacterial cells and biomolecules such as proteins by mitigating detrimental effects of environmental stresses. It was postulated that ectoine-like molecules bind to negatively charged spheres that mimic DNA surfaces. We investigated the effect of ectoine on DNA and whether ectoine is able to protect DNA from damages caused by ultraviolet radiation (UV-A). In order to determine different isoforms of DNA, agarose gel electrophoresis and atomic force microscopy experiments were carried out with plasmid pUC19 DNA. Our quantitative results revealed that a prolonged incubation of DNA with ectoine leads to an increase in transitions from supercoiled (undamaged) to open circular (single-strand break) conformation at pH 6.6. The effect is pH dependent and no significant changes were observed at physiological pH of 7.5. After UV-A irradiation in ectoine solution, changes in DNA conformation were even more pronounced and this effect was pH dependent. We hypothesize that ectoine is attracted to the negatively charge surface of DNA at lower pH and therefore fails to act as a stabilizing agent for DNA in our in vitro experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.