ArticlePloS one2017
De novo leaf and root transcriptome analysis to identify putative genes involved in triterpenoid saponins biosynthesis in Hedera helix L.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 69 citations in OpenAlex.
- A NovelCurrent genomics · 2025Article
- Functional Identification ofInternational journal of molecular sciences · 2024Article
- The genome sequence of common ivy,Wellcome open research · 2023Article
- Transcriptome analysis of three medicinal plants of the genusFrontiers in plant science · 2023Article
- Comparative transcriptomic analysis of genes in the triterpene saponin biosynthesis pathway in leaves and roots ofEcology and evolution · 2022Article
- De novo assembly and analysis of Polygonatum cyrtonema Hua and identification of genes involved in polysaccharide and saponin biosynthesis.BMC genomics · 2022Article
- Comparative transcriptome analysis of root, stem, and leaf tissues of Entada phaseoloides reveals potential genes involved in triterpenoid saponin biosynthesis.BMC genomics · 2020Article
- Identification and analysis of CYP450 and UGT supergene family members from the transcriptome of Aralia elata (Miq.) seem reveal candidate genes for triterpenoid saponin biosynthesis.BMC plant biology · 2020Article
- De novo leaf and root transcriptome analysis to explore biosynthetic pathway of Celangulin V in Celastrus angulatus maxim.BMC genomics · 2019Article
- RNA-Seq De Novo Assembly and Differential Transcriptome Analysis of Korean Medicinal Herb Cirsium japonicum var. spinossimum.Genomics & informatics · 2018Article
- Characterization of the Lycium barbarum fruit transcriptome and development of EST-SSR markers.PloS one · 2017Article
- Silverleaf Nightshade (Solanum elaeagnifolium) Undergoes Guild-Specific Transcriptomic Reprogramming Against Chewing Versus Piercing Sucking Herbivory.Physiologia plantarumArticle
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hedera helix L. is an important traditional medicinal plant in Europe. The main active components are triterpenoid saponins, but none of the potential enzymes involved in triterpenoid saponins biosynthesis have been discovered and annotated. Here is reported the first study of global transcriptome analyses using the Illumina HiSeq™ 2500 platform for H. helix. In total, over 24 million clean reads were produced and 96,333 unigenes were assembled, with an average length of 1385 nt; more than 79,085 unigenes had at least one significant match to an existing gene model. Differentially Expressed Gene analysis identified 6,222 and 7,012 unigenes which were expressed either higher or lower in leaf samples when compared with roots. After functional annotation and classification, two pathways and 410 unigenes related to triterpenoid saponins biosynthesis were discovered. The accuracy of these de novo sequences was validated by RT-qPCR analysis and a RACE clone. These data will enrich our knowledge of triterpenoid saponin biosynthesis and provide a theoretical foundation for molecular research on H. helix.
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