ArticleNature protocols2017
The Mini-FLOTAC technique for the diagnosis of helminth and protozoan infections in humans and animals.
Article in Nature protocols, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 155 papers.
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Who cites it
155 citing papers in PubMed, 327 citations in OpenAlex.
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- Therapeutic efficacy of albendazole against soil-transmitted helminthiasis in children measured by five diagnostic methods.PLoS neglected tropical diseases · 2019Trial
- Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAKG2 and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries.PLoS neglected tropical diseases · 2019Trial
- Comprehensive evaluation of stool-based diagnostic methods and benzimidazole resistance markers to assess drug efficacy and detect the emergence of anthelmintic resistance: A Starworms study protocol.PLoS neglected tropical diseases · 2018Trial
- Diagnostic comparison between FECPAKG2 and the Kato-Katz method for analyzing soil-transmitted helminth eggs in stool.PLoS neglected tropical diseases · 2018Trial
- Food contamination byFood and waterborne parasitology · 2026Article
- Environmental effects on the lung and gastrointestinal parasite fauna of wild boar: a comparative study between alpine and Mediterranean ecosystems.Acta parasitologica · 2026Article
- Field study evaluating the efficacy of a combination formulation of fluralaner with moxidectin and pyrantel (BRAVECTOParasites & vectors · 2026Article
- Comparison of wet mount, Mini-FLOTAC and Midi Parasep® coprological techniques for the detection ofInternational journal for parasitology. Parasites and wildlife · 2026Article
- Triclabendazole and Other Fasciolicides: Resistance ofAnimals : an open access journal from MDPI · 2026Review
- Parasite egg shedding rhythms are independent of feeding habits in a small shorebird host.Parasitology · 2026Article
- Life Stage-Specific Burdens and Impacts of Gastrointestinal Nematodes in Beef Cattle in the United States: A Review of Diagnostics, Impacts on Productivity, and Immune Response.Veterinary sciences · 2026Review
- Variability in the anthelmintic efficacy of levamisole against gastrointestinal nematodes of cattle, sheep and goats in South Darfur, Sudan.BMC veterinary research · 2026Article
- Development of a qPCR assay for Fasciola spp. identification and a deep amplicon sequencing method for differentiation of fluke species in UK livestock.PLoS neglected tropical diseases · 2026Article
- Increase in anthelmintic resistance in sheep flocks in Ireland.Irish veterinary journal · 2026Article
- Evaluation of the Diagnostic Performance and Validation of an AI-Assisted Fluorescence Imaging Device for Fecal Egg Counts Against the Manual McMaster Reference Method in Kiko Male Goats.Animals : an open access journal from MDPI · 2026Article
- Efficacy of prophylactic dosing of diclazuril for the prevention of coccidiosis in captive-reared North Island brown kiwi (Apteryx mantelli) chicks.Parasitology research · 2026Article
- Characterizing mixed strongyle infections in foals and broodmares using cytochrome c oxidase subunit I deep amplicon sequencing.Parasites & vectors · 2026Article
- Evaluation of OvaCyte for the detection of gastrointestinal parasites in ovine and bovine animals: comparison with traditional flotation techniques.Parasite (Paris, France) · 2026Article
95 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This protocol is an extension to: Nat. Protoc. 5, 503-515 (2010); doi: 10.1038/nprot.2009.235; published online 25 February 2010The FLOTAC is a sensitive, accurate, and precise technique for the diagnosis of protozoan and helminth infections in humans and animals. However, it requires centrifugation, and hence might be out of reach in resource-constrained settings. As an extension of the original FLOTAC protocol, this protocol describes the Mini-FLOTAC technique, a logical evolution of FLOTAC conceived to perform multivalent, qualitative, and quantitative diagnosis of helminth and protozoan infections in human and animal feces, and urine. This has been found to be of most use in the processing of large numbers of samples with rapid laboratory workup, and for veterinary applications directly on-farm. In addition to the Mini-FLOTAC apparatus, we describe the use of the Fill-FLOTAC, a closed system used to facilitate the performance of the first four consecutive steps of the Mini-FLOTAC technique: fecal sample collection and weighing, homogenization, filtration, and filling of the Mini-FLOTAC chambers. Processing of an individual sample using this protocol requires ∼12 min.
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