ArticleCellular and molecular life sciences : CMLS2018
Inorganic polyphosphate induces accelerated tube formation of HUVEC endothelial cells.
Article in Cellular and molecular life sciences : CMLS, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 46 citations in OpenAlex.
- Polyphosphate in Bone Tissue Engineering: From Molecular Mechanisms to Material Design.Journal of functional biomaterials · 2026Review
- DNA Damage and Skin Injuries Caused by Ionizing Radiation and Strategies for Wound Healing.Progress in molecular and subcellular biology · 2026Review
- Chronic Wound Healing: Breakthrough Strategies Based on Inorganic Polyphosphate.Progress in molecular and subcellular biology · 2026Review
- Platelet Polyphosphate Signals Through NFκB to Induce Myofibroblast Differentiation.Biomolecules · 2025Article
- Inorganic Polymeric Materials for Injured Tissue Repair: Biocatalytic Formation and Exploitation.Biomedicines · 2022Review
- Acceleration of chronic wound healing by bio-inorganic polyphosphate:Theranostics · 2022Article
- Polyphosphate in Chronic Wound Healing: Restoration of Impaired Metabolic Energy State.Progress in molecular and subcellular biology · 2022Article
- Effects of Polyphosphate on Leukocyte Function.Progress in molecular and subcellular biology · 2022Review
- Biomimetic Polyphosphate Materials: Toward Application in Regenerative Medicine.Progress in molecular and subcellular biology · 2022Article
- Immunoregulation and clinical significance of neutrophils/NETs-ANGPT2 in tumor microenvironment of gastric cancer.Frontiers in immunology · 2022Article
- Safety and feasibility study of using polyphosphate (PolyP) in alveolar cleft repair: a pilot study.Pilot and feasibility studies · 2021Article
- Polyphosphate (PolyP) for alveolar cleft repair: study protocol for a pilot randomized controlled trial.Trials · 2021Article
- The therapeutic potential of inorganic polyphosphate: A versatile physiological polymer to control coronavirus disease (COVID-19).Theranostics · 2021Review
- The inorganic polymer, polyphosphate, blocks binding of SARS-CoV-2 spike protein to ACE2 receptor at physiological concentrations.Biochemical pharmacology · 2020Article
- Biomimetic Alginate/Gelatin Cross-Linked Hydrogels Supplemented with Polyphosphate for Wound Healing Applications.Molecules (Basel, Switzerland) · 2020Article
- Platelet polyphosphate induces fibroblast chemotaxis and myofibroblast differentiation.Journal of thrombosis and haemostasis : JTH · 2020Article
- YB-1 transferred by gastric cancer exosomes promotes angiogenesis via enhancing the expression of angiogenic factors in vascular endothelial cells.BMC cancer · 2020Article
- Inorganic Polyphosphates As Storage for and Generator of Metabolic Energy in the Extracellular Matrix.Chemical reviews · 2019Review
- Utilization of metabolic energy in treatment of ocular surface disorders: polyphosphate as an energy source for corneal epithelial cell proliferation.RSC advances · 2019Article
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8 authors at 3 institutions in 3 countries.
Funding
Abstract
In this study, the effect of inorganic polyphosphate (polyP) on the initial phase of angiogenesis and vascularization was investigated, applying the HUVEC cell tube formation assay. PolyP is a physiological and high energy phosphate polymer which has been proposed to act as a metabolic fuel in the extracellular space with only a comparably low ATP content. The experiments revealed that polyP accelerates tube formation of human umbilical vein endothelial cells (HUVEC), seeded onto a solidified basement membrane extract matrix which contains polyP-metabolizing alkaline phosphatase (ALP) activity. This effect is abolished by co-addition of apyrase, which degrades ATP to AMP and inorganic phosphate. The assumption that ATP, derived from polyP, activates HUVEC cells leading to tube formation was corroborated by experiments showing that addition of polyP to the cells causes a strong rise of ATP level in the culture medium. Finally, we show that at a later stage of cultivation of HUVEC cells, after 3 d, polyP causes a strong enhancement of the expression of the genes encoding for the two major matrix metalloproteinases (MMPs) released by endothelial cells during tube formation, MMP-9 and MMP-2. This stimulatory effect is again abrogated by addition of apyrase together with polyP. From these results, we propose that polyP is involved either directly or indirectly in energy supply, via ALP-mediated transfer of energy-rich phosphate under ATP formation. This ATP is utilized for the activation and oriented migration of endothelial cells and for the matrix organization during the initial phases of tube formation.
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