Evidence map›Paper›PMID 28766286›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2017

Preparation of Splicing Competent Nuclear Extract from Mammalian Cells and In Vitro Pre-mRNA Splicing Assay.

Maliheh Movassat, Hossein Shenasa, Klemens J Hertel

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Maliheh MovassatDepartment of Microbiology and Molecular Genetics, University of California, Irvine, CA, USA.
Hossein ShenasaDepartment of Microbiology and Molecular Genetics, University of California, Irvine, CA, USA.
Klemens J HertelDepartment of Microbiology and Molecular Genetics, University of California, Irvine, CA, USA. khertel@uci.edu.

Funding

MECHANISMS OF ENHANCER DEPENDENT SPLICE SITE ACTIVATIONR01GM062287 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI HERTEL, KLEMENS J · 2001 to 2019
$4.8M
Tracking Gene Expression Dynamics from Transcription to DegradationR01GM110244 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI HERTEL, KLEMENS J · 2015 to 2018
$1.1M
NIGMS NIH HHS R01 GM062287NIGMS NIH HHS R01 GM110244
6 · The paper itself

Abstract

The ability to perform in vitro splicing assays has paved the way for in-depth studies of the mechanisms and machinery involved in the process of splicing. The in vitro splicing assay is a valuable experimental approach that combines the complexity of the spliceosome and regulatory systems with the flexibility of performing endless splicing and alternative splicing reactions. Through the use of crude nuclear extract and radiolabeled pre-mRNA, spliced mRNAs can be visualized using autoradiography for downstream analysis. This chapter describes the necessary steps to perform an in vitro splicing reaction, including the generation of the key components necessary for the splicing reaction; nuclear extract.

Indexed as

RNA SplicingAnimalsCell-Free SystemCell NucleusComplex MixturesHeLa CellsHumansRNA PrecursorsSpliceosomesComplex MixturesRNA PrecursorsAlternative splicingHeLa cellsIn vitro splicingIn vitro transcriptionmRNA processingNuclear extractPre-mRNA substrateRNA extraction and purificationSplicing

Identifiers

PMID28766286
PMCPMC5972008

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.