Evidence map›Paper›PMID 28766169›Full record

ArticleMolecular and cellular biochemistry2018

Retinoid acid and taurine promote NeuroD1-induced differentiation of induced pluripotent stem cells into retinal ganglion cells.

Li Huang, Mengfei Chen, Weizhong Zhang, Xuerong Sun, Bingqian Liu, Jian Ge

Abstract read
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In one paragraph

Article in Molecular and cellular biochemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Advances in Regeneration of Retinal Ganglion Cells and Optic Nerves.International journal of molecular sciences · 2021
    Review
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 6 institutions in 2 countries.

Li HuangDepartment of Ophthalmology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China.
Mengfei ChenHead&Neck Surgery Department of Otolaryngology, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA.
Weizhong ZhangOphthalmology Department, Sir Runrun Hospital Affiliated With Nanjing Medical University, Nanjing, 325200, China.
Xuerong SunInstitute of Aging Research, Dongguan Scientific Research Center, Guangdong Medical University, Dongguan, 523808, China.
Bingqian LiuState Key Laboratory of Ophthalmology, Zhongshan Ophthalmologic Center, Sun Yet-sen University, Guangzhou, 510060, China.
Jian GeState Key Laboratory of Ophthalmology, Zhongshan Ophthalmologic Center, Sun Yet-sen University, Guangzhou, 510060, China. gejian@mail.sysu.edu.cn.
First Affiliated Hospital of GuangXi Medical University · CNGuangdong Medical College · CNJohns Hopkins University · USNanjing Medical University · CNState Key Laboratory of OphthalmologySun Yat-sen University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Induced pluripotent stem cells (iPSCs) possess the capacity to differentiate into multiple cell types including retinal neurons. Despite substantial progress in the transcriptional regulation of iPSC differentiation process, the efficiency of generation of retinal neurons from iPSCs is still low. In this study, we investigated the role of transcription factor NeuroD1 in the differentiation of iPSCs into retinal neurons. We observed that retrovirus-mediated NeuroD1 overexpression in iPSCs increased the efficiency of neuronal differentiation. Immunostaining analysis showed that NeuroD1 overexpression increased the expression of retina ganglion cell markers including Islet-1, Math5, Brn3b, and Thy1.2. Retinoid acid (RA) and taurine further improved the differentiation efficiency of iPSCs overexpressing NeuroD1. However, RA and taurine did not promote differentiation in the absence of NeuroD1 overexpression. Together, our study provides new evidence in transcription factor-regulated stem cell differentiation in vitro.

Indexed as

AnimalsBasic Helix-Loop-Helix ProteinsCell DifferentiationInduced Pluripotent Stem CellsMiceMice, Inbred BALB CRetinal Ganglion CellsTaurineTretinoinBasic Helix-Loop-Helix ProteinsNeurod1 protein, mouseTaurineTretinoinNeuroD1Pluripotent stem cellsRetinal ganglion cellsRetinoic acidTaurine

Identifiers

PMID28766169
OpenAlexW2741618668

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.