ArticleScientific reports2017
CD14 is critical for TLR2-mediated M1 macrophage activation triggered by N-glycan recognition.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 74 citations in OpenAlex.
- The cell with many faces: lung macrophage plasticity and function in response to environmental and pathogenic insults.Physiological reviews · 2026Review
- RNA Sequence Analysis in Macrophages Infected With Trypanosoma cruzi: Focus on TLR2 and TLR7, Iron Metabolism, and Extracellular Matrix Biosynthesis.The Journal of infectious diseases · 2025Article
- Investigations of membrane protein interactions in cells using fluorescence microscopy.Current opinion in structural biology · 2024Review
- Causal relationship between immune cells and hepatocellular carcinoma: a Mendelian randomisation study.Journal of Cancer · 2024Article
- Seasonal influenza vaccines differentially activate and modulate toll-like receptor expression within the tumor microenvironment.Frontiers in oncology · 2024Article
- The obese inflammatory microenvironment may promote breast DCIS progression.Frontiers in immunology · 2024Article
- Adjuvant ArtinM favored the host immunity againstImmunotherapy · 2024Article
- Role of pattern recognition receptors in sensingHeliyon · 2023Review
- An Update on Toll-like Receptor 2, Its Function and Dimerization in Pro- and Anti-Inflammatory Processes.International journal of molecular sciences · 2023Review
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- Aggregated alpha-synuclein transcriptionally activates pro-inflammatory canonical and non-canonical NF-κB signaling pathways in peripheral monocytic cells.Molecular immunology · 2023Article
- ArtinM Cytotoxicity in B Cells Derived from Non-Hodgkin's Lymphoma Depends on Syk and Src Family Kinases.International journal of molecular sciences · 2023Article
- The macrophage infectivity potentiator ofFrontiers in immunology · 2023Article
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- Expression of Antimicrobic Peptide Piscidin1 in Gills Mast Cells of Giant MudskipperInternational journal of molecular sciences · 2022Article
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- CD14+ monocytes and soluble CD14 of synovial fluid are associated with osteoarthritis progression.Archives of rheumatology · 2022Article
- Lactobacillus rhamnosus Affects Rat Peritoneal Cavity Cell Response to Stimulation with Gut Microbiota: Focus on the Host Innate Immunity.Inflammation · 2021Article
- The Transcription Factor SUB1 Is a Master Regulator of the Macrophage TLR Response in Atherosclerosis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Agonist interaction with Toll-like receptors (TLRs) induces T cell-mediated immunity, which is effective against intracellular pathogens. Consequently, TLR agonists are being tried as immunomodulatory agents. The lectin ArtinM targets TLR2 N-glycans on macrophages, induces cytokines production, and promotes T helper-1 immunity, a process that culminates in resistance to several parasitic and fungal infections in vivo. Because co-receptors influence agonist binding to TLRs, we investigated whether CD14 is required for macrophage activation induced by ArtinM. Macrophages from wild-type mice stimulated by ArtinM not only produced cytokines but also had the following activation profile: (i) expression of M1 polarization markers; (ii) nitrite oxide production; (iii) cellular migration; (iv) enhanced phagocytic and fungicide activity; (v) modulation of TLR2 expression; and (vi) activation of NF-κB pathway. This activation profile induced by ArtinM was evaluated in macrophages lacking CD14 that showed none of the ArtinM effects. We demonstrated by immunoprecipitation and sugar inhibition assays the physical interaction of ArtinM, TLR2, and CD14, which depends on recognition of the trimannoside that constitutes the core of N-glycans. Thus, our study showed that CD14 is critical for ArtinM-induced macrophage activation, providing fundamental insight into the design of anti-infective therapies based on carbohydrate recognition.
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