ArticleScientific reports2017
Non-peptide guided auto-secretion of recombinant proteins by super-folder green fluorescent protein in Escherichia coli.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 51 citations in OpenAlex.
- Superfolder Green Fluorescent Protein (sfGFP): A Versatile Tool for Biotechnology Research.Applied biochemistry and biotechnology · 2026Review
- Establishing a NovelBiomolecules · 2025Article
- Visual and High-Efficiency Secretion of SARS-CoV-2 Nanobodies withBiomolecules · 2025Article
- Cell factories for biosynthesis of D-glucaric acid: a fusion of static and dynamic strategies.World journal of microbiology & biotechnology · 2024Review
- A Whole-Process Visible Strategy for the Preparation of Rhizomucor miehei Lipase with Escherichia coli Secretion Expression System and the Immobilization.Microbial cell factories · 2024Article
- Engineering of Unspecific Peroxygenases Using a Superfolder-Green-Fluorescent-Protein-Mediated Secretion System inJACS Au · 2024Article
- Whole-Cell Display of Phospholipase D inBiomolecules · 2024Article
- Biological Function of Prophage-Related Gene Cluster ΔInternational journal of molecular sciences · 2024Article
- Export of Diverse and Bioactive Small Proteins through a Type I Secretion System.Applied and environmental microbiology · 2023Article
- Cloning and Characterization of Fructose-1,6-Bisphosphate Aldolase fromInternational journal of molecular sciences · 2022Article
- Whole-Cell Display of Phosphotransferase inBiomolecules · 2022Article
- Encapsulating IM7-Displaying Yeast Cells in Calcium Alginate Beads for One-Step Protein Purification and Multienzyme Biocatalysis.Frontiers in bioengineering and biotechnology · 2022Article
- DSResSol: A Sequence-Based Solubility Predictor Created with Dilated Squeeze Excitation Residual Networks.International journal of molecular sciences · 2021Article
- Cadaverine Production From L-Lysine With Chitin-Binding Protein-Mediated Lysine Decarboxylase Immobilization.Frontiers in bioengineering and biotechnology · 2020Article
- Aliivibrio wodanis as a production host: development of genetic tools for expression of cold-active enzymes.Microbial cell factories · 2019Article
- Flavin-Based Fluorescent Protein EcFbFP Auto-Guided Surface Display of Methyl Parathion Hydrolase in Escherichia coli.Molecular biotechnology · 2019Article
- A Highly Efficient IndirectMolecules (Basel, Switzerland) · 2019Article
- Developing Gram-negative bacteria for the secretion of heterologous proteins.Microbial cell factories · 2018Review
- Toward Light-Regulated Living Biomaterials.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein secretion in Escherichia coli is usually led by a signal peptide that targets the protein to specific secretory pathways. In this study, we demonstrated that the superfolder green fluorescent protein (sfGFP) could be served as a non-signal peptide to guide protein auto-secretion in E. coli. This auto-secretion was characterized as a three-step process through the sub-cellular localization analysis: inner membrane trans-location followed by anchoring at outer membrane, and then being released into culture media. We further determined that the beta-barrel structure and net negative charges of sfGFP played important roles in its auto-extracellular secretion property. Using sfGFP as a carrier, heterologous proteins ranging from peptide to complex protein, including antibacterial peptide PG4, endo-beta-N-acethylglucosamindase H (Endo H), human arginase-1 (ARG1), and glutamate decarboxylase (GAD) were all successfully expressed and secreted extracellularly when fused to the carboxyl end of sfGFP. Besides facilitating the extracellular secretion, sfGFP fusion proteins can also be correctly folded and formed the active complex protein structure, including the trimetric human ARG1 and homo-hexametric GAD. This is the first report that sfGFP can guide the secretion of recombinant proteins out of the cells from cytoplasm in E. coli without affecting their conformation and function.
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