Evidence map›Paper›PMID 28764802›Full record

ArticleStem cell research & therapy2017

Mesenchymal stem cells derived from inflamed dental pulpal and gingival tissue: a potential application for bone formation.

Laura Tomasello, Rodolfo Mauceri, Antonina Coppola, Maria Pitrone, Giuseppe Pizzo, Giuseppina Campisi, Giuseppe Pizzolanti, Carla Giordano

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
56citing papers in PubMed, 1 pooled it
6.0field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

56 citing papers in PubMed, 1 synthesis or guideline pooled it, 100 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Article
  4. Gingival Mesenchymal Stem Cells: A Periodontal Regenerative Substitute.Tissue engineering and regenerative medicine · 2025
    Review
  5. Review
  6. Review
  7. Article
  8. Review
  9. Article
  10. Heat Shock Proteins in Tooth Development and Injury Repair.International journal of molecular sciences · 2023
    Review
  11. Review
  12. Review
  13. Review
  14. Article
  15. Review
  16. Article
  17. Article
  18. Article
  19. Article
  20. Role of Hydrogen Sulfide in Oral Disease.Oxidative medicine and cellular longevity · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Laura TomaselloLaboratory of Regenerative Medicine "Aldo Galluzzo", Department of Endocrinology, Diabetology and Metabolism, University of Palermo, Piazza Delle Cliniche 2, 90127, Palermo, Italy.
Rodolfo MauceriDepartment of Surgical, Oncological and Oral Sciences, University of Palermo, Palermo, Italy.
Antonina CoppolaLaboratory of Regenerative Medicine "Aldo Galluzzo", Department of Endocrinology, Diabetology and Metabolism, University of Palermo, Piazza Delle Cliniche 2, 90127, Palermo, Italy.
Maria PitroneLaboratory of Regenerative Medicine "Aldo Galluzzo", Department of Endocrinology, Diabetology and Metabolism, University of Palermo, Piazza Delle Cliniche 2, 90127, Palermo, Italy.
Giuseppe PizzoDepartment of Surgical, Oncological and Oral Sciences, University of Palermo, Palermo, Italy.
Giuseppina CampisiDepartment of Surgical, Oncological and Oral Sciences, University of Palermo, Palermo, Italy.
Giuseppe PizzolantiLaboratory of Regenerative Medicine "Aldo Galluzzo", Department of Endocrinology, Diabetology and Metabolism, University of Palermo, Piazza Delle Cliniche 2, 90127, Palermo, Italy.
Carla GiordanoLaboratory of Regenerative Medicine "Aldo Galluzzo", Department of Endocrinology, Diabetology and Metabolism, University of Palermo, Piazza Delle Cliniche 2, 90127, Palermo, Italy. carla.giordano@unipa.it.
University of Palermo · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundChronic periodontal disease is an infectious disease consisting of prolonged inflammation of the supporting tooth tissue and resulting in bone loss. Guided bone regeneration procedures have become common and safe treatments in dentistry, and in this context dental stem cells would represent the ideal solution as autologous cells. In this study, we verified the ability of dental pulp mesenchymal stem cells (DPSCs) and gingival mesenchymal stem cells (GMSCs) harvested from periodontally affected teeth to produce new mineralized bone tissue in vitro, and compared this to cells from healthy teeth.

methodsTo characterize DPSCs and GMSCs, we assessed colony-forming assay, immunophenotyping, mesenchymal/stem cell phenotyping, stem gene profiling by means of flow cytometry, and quantitative polymerase chain reaction (qPCR). The effects of proinflammatory cytokines on mesenchymal stem cell (MSC) proliferation and differentiation potential were investigated. We also observed participation of several heat shock proteins (HSPs) and actin-depolymerizing factors (ADFs) during osteogenic differentiation.

resultsDPSCs and GMSCs were successfully isolated both from periodontally affected dental tissue and controls. Periodontally affected dental MSCs proliferated faster, and the inflamed environment did not affect MSC marker expressions. The calcium deposition was higher in periodontally affected MSCs than in the control group. Proinflammatory cytokines activate a cytoskeleton remodeling, interacting with HSPs including HSP90 and HSPA9, thioredoxin-1, and ADFs such as as profilin-1, cofilin-1, and vinculin that probably mediate the increased acquisition in the inflamed environment.

conclusionsOur findings provide evidence that periodontally affected dental tissue (both pulp and gingiva) can be used as a source of MSCs with intact stem cell properties. Moreover, we demonstrated that the osteogenic capability of DPSCs and GMSCs in the test group was not only preserved but increased by the overexpression of several proinflammatory cytokine-dependent chaperones and stress response proteins.

Indexed as

Cell DifferentiationOsteogenesisAdolescentAdultAgedCells, CulturedDental PulpFemaleGingivaGingivitisHumansMaleMesenchymal Stem CellsMiddle AgedPeriodontitisADFsBone formationDental diseaseHeat shock proteinInflammationProinflammatory cytokinesPulpal and gingival mesenchymal stem cells

Identifiers

PMID28764802
PMCPMC5540218
OpenAlexW2741474860

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.