ArticleBMC infectious diseases2017
Invasive anisakiasis by the parasite Anisakis pegreffii (Nematoda: Anisakidae): diagnosis by real-time PCR hydrolysis probe system and immunoblotting assay.
Article in BMC infectious diseases, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 35 citations in OpenAlex.
- Experimental pathogenicity ofFood and waterborne parasitology · 2026Article
- Anisakiasis: A Decade of Molecular and Diagnostic Advancements (2015-2026).International journal of molecular sciences · 2026Review
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- Prevalence of fish parasites from Lake Hawassa, Ethiopia.PloS one · 2026Article
- Gastric Submucosal Tumor in Patient Infected with Dioctophyme renale Roundworm, South Korea, 2024.Emerging infectious diseases · 2025Article
- Seroprevalence and risk factors of anisakiasis associated with raw seafood consumption in the Sangihe Islands, Indonesia.Journal of advanced veterinary and animal research · 2025Article
- Redescription of alien nematode,Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2024Article
- Re-evaluation of certain aspects of the EFSA Scientific Opinion of April 2010 on risk assessment of parasites in fishery products, based on new scientific data. Part 1: ToRs1-3.EFSA journal. European Food Safety Authority · 2024Article
- Basophil Activation Test in the Diagnosis ofPathogens (Basel, Switzerland) · 2023Article
- Parasite Community Analysis of The Gray SnapperHelminthologia · 2022Article
- Preliminary Data on the Occurrence ofMicroorganisms · 2022Article
- Article
- Fifty-millimeter abscess in the ileum caused by perforation from anisakiasis successfully treated with conservative therapy without drainage.Oxford medical case reports · 2021Article
- A Comprehensive Review of Infectious Granulomatous Diseases of the Gastrointestinal Tract.Gastroenterology research and practice · 2021Review
- A Critical Appraisal of Global Testing Protocols for Zoonotic Parasites in Imported Seafood Applied to Seafood Safety in Australia.Foods (Basel, Switzerland) · 2020Review
- Immune response evaluation inVeterinary world · 2019Article
- Article
- Gene expression profiles of antigenic proteins of third stage larvae of the zoonotic nematode Anisakis pegreffii in response to temperature conditions.Parasite (Paris, France) · 2019Article
- Molecular and Cellular Response to ExperimentalFrontiers in immunology · 2018Article
- Human anisakiasis in Italy: a retrospective epidemiological study over two decades.Parasite (Paris, France) · 2018Review
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10 authors at 5 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundAnisakiasis is a fish-borne zoonosis caused by Anisakis spp. larvae. One challenging issue in the diagnosis of anisakiasis is the molecular detection of the etiological agent even at very low quantity, such as in gastric or intestinal biopsy and granulomas. Aims of this study were: 1) to identify three new cases of invasive anisakiasis, by a species-specific Real-time PCR probe assay; 2) to detect immune response of the patients against the pathogen.
methodsParasite DNA was extracted from parasites removed in the three patients. The identification of larvae removed at gastric and intestinal level from two patients was first obtained by sequence analysis of mtDNA cox2 and EF1 α-1 of nDNA genes. This was not possible in the third patient, because of the very low DNA quantity obtained from a single one histological section of a surgically removed granuloma. Real-time PCR species-specific hydrolysis probe system, based on mtDNA cox2 gene, was performed on parasites tissue of the three cases. IgE, IgG
resultsAccording to the mtDNA cox2 and the EF1 α - 1 nDNA sequence analysis, the larvae from stomach and intestine of two patients were assigned to A. pegreffii. The Real-time PCR primers/probe system, showed a fluorescent signal at 510 nm for A. pegreffii, in all the three cases. In Immunoblotting assay, patient CC1 showed IgE, IgG
conclusionThe Real-time PCR assay, a more sensitive method than direct DNA sequencing for the accurate and rapid identification of etiological agent of human anisakiasis, was successfully assessed for the first time. The study also highlights the importance to use both molecular and immunological tools in the diagnosis of human anisakiasis, in order to increase our knowledge about the pathological findings and immune response related to the infection by zoonotic species of the genus Anisakis.
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