Evidence map›Paper›PMID 28763567›Full record

ArticleNeurourology and urodynamics2018

Low-intensity extracorporeal shock wave therapy promotes myogenesis through PERK/ATF4 pathway.

Bohan Wang, Jun Zhou, Lia Banie, Amanda B Reed-Maldonado, Hongxiu Ning, Zhihua Lu, Yajun Ruan, Tie Zhou, Hsun Shuan Wang, Byung Seok Oh and 4 more

Registry-linked trialOpen access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neurourology and urodynamics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04161183 (Extracoporeal Shock Wave Therapy), which is not on this map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
2.6field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT04161183 nacompletednot on this mapstarted 2019, after this paper: background citation

Extracoporeal Shock Wave Therapy (ESWT) for the Treatment of Detrusor Underactivity/ Underactive Bladder (DU/UAB) - a Single Center, Randomized, Double-blind, Placebo-controlled, Prospective Study

TypeinterventionalSponsorChang Gung Memorial HospitalRan2019 to 2022Enrolled11ConditionsDetrusor, Underactive, Detrusor UnderactivityArmsExtracoporeal shock wave therapy, Placebo
3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 43 citations in OpenAlex.

  1. Trial
  2. Trial
  3. Trial
  4. Article
  5. Article
  6. Article
  7. Review
  8. Review
  9. Article
  10. Review
  11. Article
  12. Article
  13. Microenergy acoustic pulses promotes muscle regeneration through in situ activation of muscle stem cells.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2022
    Article
  14. Article
  15. Review
  16. Tissue-engineered repair material for pelvic floor dysfunction.Frontiers in bioengineering and biotechnology · 2022
    Review
  17. Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors at 2 institutions in 2 countries.

Bohan WangKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California.ORCID 0000-0003-3561-9367
Jun ZhouKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California.
Lia BanieKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California.
Amanda B Reed-MaldonadoKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California.
Hongxiu NingKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California.
Zhihua LuKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California.
Yajun RuanKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California.
Tie ZhouKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California.
Hsun Shuan WangKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California.
Byung Seok OhKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California.
Guifang WangKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California.
Stanley Lei QiDepartment of Bioengineering, Stanford University, Stanford, California.
Guiting LinKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California.
Tom F LueKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California.
University of California, San Francisco · USStanford University · US

Funding

Therapy for Obesity-associated Stress Urinary IncontinenceR01DK105097 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LUE, TOM F · 2016 to 2019
$2.6M
Therapy for Obesity-associated Stress Urinary IncontinenceR56DK105097 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LUE, TOM F · 2015 to 2015
$100k
NIDDK NIH HHS R01 DK105097NIDDK NIH HHS R56 DK105097
6 · The paper itself

Abstract

aimStress urinary incontinence (SUI) is a significant health problem for women. Treatments employing muscle derived stem cells (MDSCs) may be a promising approach to this prevalent, bothersome condition, but these treatments are invasive and require collection of cells from one site for injection into another. It is also unknown whether or not these cells establish themselves and function as muscle cells in the target tissues. Alternatively, low-intensity extracorporeal shock wave therapy (Li-ESWT) is non-invasive and has shown positive outcomes in the treatment of multiple musculoskeletal disorders, but the biological effects responsible for clinical success are not yet well understood. The aim of this study is to explore the possibility of employing Li-ESWT for activation of MDSCs in situ and to further elucidate the underlying biological effects and mechanisms of action in urethral muscle.

methodsUrethral muscle derived stem cells (uMDSCs) were harvest from Zucker Lean (ZUC-LEAN) (ZUC-Leprfa 186) rats and characterized with flow cytometry. Li-ESWT (0.02 mJ/mm

resultsThe results indicate that uMDSCs could form myotubes. Myotube formation was significantly increased by the Li-ESWT as was the expression of muscle heavy chain (MHC) and myogenic factor 5 (Myf5) in L6 cells in vitro. Li-ESWT activated protein kinase RNA-like ER kinase (PERK) pathway by increasing the phosphorylation levels of PERK and eukaryotic initiation factor 2a (eIF2α) and by increasing activating transcription factor 4 (ATF4). In addition, GSK2656157, an inhibitor of PERK, effectively inhibited the myotube formation in L6 rat myoblast cells. Furthermore, GSK2656157 also attenuated myotube formation induced by Li-ESWT.

conclusionIn conclusion, this experiment reveals that rat uMDSCs can be isolated successfully and can form myotubes in vitro. PERK/ATF4 pathway was involved in myotube formation, and L6 rat myoblast cells were activated by Li-ESWT to form myotubes. These findings suggest that PERK/ATF4 pathway is activated by Li-ESWT. This study elucidates one of the biochemical pathways responsible for the clinical improvements seen after Li-ESWT. It is possible that this information will help to establish Li-ESWT as an acceptable treatment modality and may help to further refine the use of Li-ESWT in the clinical practice of medicine.

Indexed as

Extracorporeal Shockwave TherapyActivating Transcription Factor 4AdenineAnimalseIF-2 KinaseEukaryotic Initiation Factor-2IndolesMuscle DevelopmentMyoblastsPhosphorylationRatsRats, ZuckerSignal TransductionStem CellsActivating Transcription Factor 4AdenineeIF-2 KinaseEukaryotic Initiation Factor-2GSK2656157Indoleslow-intensity extracorporeal shock wave treatmentmyogenesisPERK/ATF4 pathwayurethral muscle derived stem cells

Identifiers

PMID28763567
PMCPMC5794657
OpenAlexW2741830607

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.