Evidence map›Paper›PMID 28763561›Full record

ArticleInvestigative ophthalmology & visual science2017

Establishment and Characterization of an Acute Model of Ocular Hypertension by Laser-Induced Occlusion of Episcleral Veins.

Liwei Zhang, Guangyu Li, Meng Shi, Hsin-Hua Liu, Shaokui Ge, Yvonne Ou, John G Flanagan, Lu Chen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Investigative ophthalmology & visual science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.1field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 17 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Organ Cultures for Retinal Diseases.Frontiers in neuroscience · 2020
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Liwei ZhangCenter for Eye Disease and Development, Vision Science Graduate Program, University of California, Berkeley, California, United States 2School of Optometry and Vision Science, University of California, Berkeley, California, United States 3Department of Ophthalmology, Second Xiangya Hospital, Central South University, Changsha, China.
Guangyu LiCenter for Eye Disease and Development, Vision Science Graduate Program, University of California, Berkeley, California, United States 2School of Optometry and Vision Science, University of California, Berkeley, California, United States.
Meng ShiCenter for Eye Disease and Development, Vision Science Graduate Program, University of California, Berkeley, California, United States 2School of Optometry and Vision Science, University of California, Berkeley, California, United States 3Department of Ophthalmology, Second Xiangya Hospital, Central South University, Changsha, China.
Hsin-Hua LiuCenter for Eye Disease and Development, Vision Science Graduate Program, University of California, Berkeley, California, United States 2School of Optometry and Vision Science, University of California, Berkeley, California, United States.
Shaokui GeCenter for Eye Disease and Development, Vision Science Graduate Program, University of California, Berkeley, California, United States 2School of Optometry and Vision Science, University of California, Berkeley, California, United States.
Yvonne OuDepartment of Ophthalmology, University of California, San Francisco, California, United States.
John G FlanaganCenter for Eye Disease and Development, Vision Science Graduate Program, University of California, Berkeley, California, United States 2School of Optometry and Vision Science, University of California, Berkeley, California, United States.
Lu ChenCenter for Eye Disease and Development, Vision Science Graduate Program, University of California, Berkeley, California, United States 2School of Optometry and Vision Science, University of California, Berkeley, California, United States.
University of California, Berkeley · USUniversity of California, San Francisco · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Purpose: This study was designed to develop and characterize a laser-induced model of acute intraocular hypertension that permits the study of the anterior segment of the eye. Methods: CD1 mice aged 5 and 8 weeks were examined for elevation of IOP induced by laser photocoagulation. We compared between occlusion of episcleral veins alone and when combined with 270° limbal vessel occlusion. Anterior chamber angle, corneal thickness, and retinal nerve fiber layer (RNFL) thickness were evaluated by anterior- and posterior-segment optical coherence tomography (OCT). Additionally, at day 7 post-procedure, the anterior segment was evaluated for inflammatory cellular presentation by histologic analysis and OCT, and limbal vessels and whole-mount retina were immunostained for CD31 and Brn3a, respectively. Brn3a-positive retinal ganglion cells (RGCs) were quantified with ImageJ software. Results: After single or combined laser treatment in mice aged 5 or 8 weeks, IOP was significantly elevated for 5 to 6 days before returning to the baseline by day 7 post-procedure. Anterior segment assessment indicated less synechiae in the anterior chamber angle and better preserved limbal vessels with single versus combined laser treatment. Corneal thickness was significantly increased after single or combined treatment. No inflammatory cells were detected in the anterior chamber. The thickness of the RNFL and the density of RGCs were both significantly reduced after single or combined treatment. Conclusions: Laser photocoagulation of episcleral veins alone in CD1 mice aged 5 to 8 weeks may be used to induce ocular hypertension resulting in RNFL thinning and ganglion cell loss. This model permits the study of the anterior as well as the posterior segment of the eye.

Indexed as

Acute DiseaseAnimalsDisease Models, AnimalIntraocular PressureLight CoagulationLimbus CorneaeOcular HypertensionOptic DiskRetinaRetinal Ganglion CellsRetinal Vein OcclusionScleraTomography, Optical CoherenceVeins

Identifiers

PMID28763561
PMCPMC6108309
OpenAlexW2738269054

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.