ArticlePloS one2017
Global assessment of small RNAs reveals a non-coding transcript involved in biofilm formation and attachment in Acinetobacter baumannii ATCC 17978.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 23 citations in OpenAlex.
- Dissecting the essential role ofVirulence · 2026Article
- Small regulatory RNAs mediated regulation of virulence and host-pathogen interaction in the Gram-negative ESKAPE pathogens.Virulence · 2026Review
- Identification and differential expression analysis of Hfq-bound novel small RNAs in Serratia marcescens by nitrocellulose-based partitioning.Molecular biology reports · 2026Article
- Nanoemulsion-based colistin for pulmonary delivery: Enhanced antibacterial efficacy against Acinetobacter baumannii.Drug delivery and translational research · 2026Article
- Inside Enemy Lines: Adhesion, Invasion, and Intracellular Persistence ofPathogens (Basel, Switzerland) · 2026Review
- Article
- Growth and Polarization of A549 Human Alveolar Epithelial Cells as a Model to Study Biofilm Formation in Acinetobacter baumannii.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Recent advances in host models in studying virulence factors and pathogenesis ofFrontiers in cellular and infection microbiology · 2025Review
- 'GGFGGQ' repeats in Hfq of Acinetobacter baumannii are essential for nutrient utilization and virulence.The Journal of biological chemistry · 2024Article
- Insights into the regulatory role of bacterial sncRNA and its extracellular delivery via OMVs.Applied microbiology and biotechnology · 2024Review
- Global analysis of the RNA-RNA interactome in Acinetobacter baumannii AB5075 uncovers a small regulatory RNA repressing the virulence-related outer membrane protein CarO.Nucleic acids research · 2024Article
- Regulation of biofilm formation by non-coding RNA in prokaryotes.Current research in pharmacology and drug discovery · 2023Review
- Transcriptome Analysis ofInternational journal of environmental research and public health · 2022Article
- TheFrontiers in molecular biosciences · 2022Review
- Modulation of Quorum Sensing and Biofilms in Less Investigated Gram-Negative ESKAPE Pathogens.Frontiers in microbiology · 2021Review
- Small noncoding RNA sRNA0426 is involved in regulating biofilm formation in Streptococcus mutans.MicrobiologyOpen · 2020Article
- Regulatory networks important for survival of Acinetobacter baumannii within the host.Current opinion in microbiology · 2020Review
- Insights Into Mechanisms of Biofilm Formation inFrontiers in cellular and infection microbiology · 2020Review
- COLFrontiers in microbiology · 2019Article
- Review
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many strains of Acinetobacter baumannii have been described as being able to form biofilm. Small non-coding RNAs (sRNAs) control gene expression in many regulatory circuits in bacteria. The aim of the present work was to provide a global description of the sRNAs produced both by planktonic and biofilm-associated (sessile) cells of A. baumannii ATCC 17978, and to compare the corresponding gene expression profiles to identify sRNAs molecules associated to biofilm formation and virulence. sRNA was extracted from both planktonic and sessile cells and reverse transcribed. cDNA was subjected to 454-pyrosequencing using the GS-FLX Titanium chemistry. The global analysis of the small RNA transcriptome revealed different sRNA expression patterns in planktonic and biofilm associated cells, with some of the transcripts only expressed or repressed in sessile bacteria. A total of 255 sRNAs were detected, with 185 of them differentially expressed in the different types of cells. A total of 9 sRNAs were expressed only in biofilm cells, while the expression of other 21 coding regions were repressed only in biofilm cells. Strikingly, the expression level of the sRNA 13573 was 120 times higher in biofilms than in planktonic cells, an observation that prompted us to further investigate the biological role of this non-coding transcript. Analyses of an isogenic mutant and over-expressing strains revealed that the sRNA 13573 gene is involved in biofilm formation and attachment to A549 human alveolar epithelial cells. The present work serves as a basis for future studies examining the complex regulatory network that regulate biofilm biogenesis and attachment to eukaryotic cells in A. baumannii ATCC 17978.
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