ReviewBiophysical reviews2017
Directly watching biomolecules in action by high-speed atomic force microscopy.
Review in Biophysical reviews, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 44 citations in OpenAlex.
- High-speed atomic force microscopy of membrane proteins: From dynamic imaging to integrative structural biology.Current opinion in structural biology · 2026Review
- Viruses under mechanical force: Implications and applications.Virus research · 2026Review
- Insight into Single-Molecule Imaging Techniques for the Study of Prokaryotic Genome Maintenance.Chemical & biomedical imaging · 2024Review
- Development of Single Molecule Techniques for Sensing and Manipulation of CRISPR and Polymerase Enzymes.Small (Weinheim an der Bergstrasse, Germany) · 2023Review
- Physical Properties and Shifting of the Extracellular Membrane Vesicles Attached to Living Bacterial Cell Surfaces.Microbiology spectrum · 2022Article
- A Single Shot of Vesicles.Microbes and environments · 2022Article
- Practical considerations for feature assignment in high-speed AFM of live cell membranes.Biophysics and physicobiology · 2022Article
- An ultra-wide scanner for large-area high-speed atomic force microscopy with megapixel resolution.Scientific reports · 2021Article
- Peroxiredoxins wear many hats: Factors that fashion their peroxide sensing personalities.Redox biology · 2021Review
- Scanning Probe Microscopies: Imaging and Biomechanics in Reproductive Medicine Research.International journal of molecular sciences · 2021Review
- Biological physics by high-speed atomic force microscopy.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2020Review
- High-speed atomic force microscopy highlights new molecular mechanism of daptomycin action.Nature communications · 2020Article
- Combining Experimental Data and Computational Methods for the Non-Computer Specialist.Molecules (Basel, Switzerland) · 2020Review
- Mechanotransduction, nanotechnology, and nanomedicine.Journal of biomedical research · 2020Article
- Visualization of Single Molecules Building a Viral Capsid Protein Lattice through Stochastic Pathways.ACS nano · 2020Article
- Quantifying single-platelet biomechanics: An outsider's guide to biophysical methods and recent advances.Research and practice in thrombosis and haemostasis · 2020Review
- Using Atomic Force Microscopy To Illuminate the Biophysical Properties of Microbes.ACS applied bio materials · 2020Article
- Mechanical Characterization of Liposomes and Extracellular Vesicles, a Protocol.Frontiers in molecular biosciences · 2020Article
- Simultaneous Quantification of the Interplay Between Molecular Turnover and Cell Mechanics by AFM-FRAP.Small (Weinheim an der Bergstrasse, Germany) · 2019Article
- Coarse-Grained Protein Dynamics Studies Using Elastic Network Models.International journal of molecular sciences · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Proteins are dynamic in nature and work at the single molecule level. Therefore, directly watching protein molecules in dynamic action at high spatiotemporal resolution must be the most straightforward approach to understanding how they function. To make this observation possible, high-speed atomic force microscopy (HS-AFM) has been developed. Its current performance allows us to film biological molecules at 10-16 frames/s, without disturbing their function. In fact, dynamic structures and processes of various proteins have been successfully visualized, including bacteriorhodopsin responding to light, myosin V walking on actin filaments, and even intrinsically disordered proteins undergoing order/disorder transitions. The molecular movies have provided insights that could not have been reached in other ways. Moreover, the cantilever tip can be used to manipulate molecules during successive imaging. This capability allows us to observe changes in molecules resulting from dissection or perturbation. This mode of imaging has been successfully applied to myosin V, peroxiredoxin and doublet microtubules, leading to new discoveries. Since HS-AFM can be combined with other techniques, such as super-resolution optical microscopy and optical tweezers, the usefulness of HS-AFM will be further expanded in the near future.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.