Evidence map›Paper›PMID 28762033›Full record

ArticleBiotechnology letters2017

Inducible somatic embryogenesis in Theobroma cacao achieved using the DEX-activatable transcription factor-glucocorticoid receptor fusion.

Morgan E Shires, Sergio L Florez, Tina S Lai, Wayne R Curtis

Expression of concernAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology letters, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It carries an expression of concern. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

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  7. Genotype-independent plant transformation.Horticulture research · 2022
    Article
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  10. Maize transformation: history, progress, and perspectives.Molecular breeding : new strategies in plant improvement · 2021
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors.

Morgan E ShiresDepartment of Chemical Engineering, The Pennsylvania State University, University Park, PA, 16802-4400, USA.
Sergio L FlorezDepartment of Chemical Engineering, The Pennsylvania State University, University Park, PA, 16802-4400, USA.
Tina S LaiDepartment of Chemical Engineering, The Pennsylvania State University, University Park, PA, 16802-4400, USA.
Wayne R CurtisDepartment of Chemical Engineering, The Pennsylvania State University, University Park, PA, 16802-4400, USA. wrc2@psu.edu.

Funding

Directorate for Biological Sciences 1543929National Science Foundation 1035072
6 · The paper itself

Abstract

objectivesTo carry out mass propagation of superior plants to improve agricultural and silvicultural production though advancements in plant cell totipotency, or the ability of differentiated somatic plant cells to regenerate an entire plant.

resultsThe first demonstration of a titratable control over somatic embryo formation in a commercially relevant plant, Theobroma cacao (Chocolate tree), was achieved using a dexamethasone activatable chimeric transcription factor. This four-fold enhancement in embryo production rate utilized a glucocorticoid receptor fused to an embryogenic transcription factor LEAFY COTYLEDON 2. Where previous T. cacao somatic embryogenesis has been restricted to dissected flower parts, this construct confers an unprecedented embryogenic potential to leaves.

conclusionsActivatable chimeric transcription factors provide a means for elucidating the regulatory cascade associated with plant somatic embryogenesis towards improving its use for somatic regeneration of transgenics and plant propagation.

Indexed as

CacaoDexamethasoneEnzyme ActivationGene Expression Regulation, DevelopmentalGene Expression Regulation, PlantPlant ProteinsPlants, Genetically ModifiedPlant Somatic Embryogenesis TechniquesReceptors, GlucocorticoidRecombinant Fusion ProteinsTranscription FactorsDexamethasonePlant ProteinsReceptors, GlucocorticoidRecombinant Fusion ProteinsTranscription FactorsChimeric transcription factorChocolateGlucocorticoid receptorLEAFY COTYLEDON 2Plant propagationSomatic embryogenesisTheobroma cacao

Identifiers

PMID28762033
PMCPMC5636861

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.