ArticlePeerJ2017
DNA-barcoding of forensically important blow flies (Diptera: Calliphoridae) in the Caribbean Region.
Article in PeerJ, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Considerations for multivariate approaches to classify blow fly species based on cuticular hydrocarbon profiles.Journal of forensic sciences · 2026Article
- DNA Recovery from Forensically Relevant Blow Fly Larvae (Insecta, Diptera, Calliphoridae) Kept in Different Preservative Solutions.Neotropical entomology · 2026Article
- Article
- Myiasis in European hedgehogs (The veterinary quarterly · 2025Article
- Article
- Molecular identification of immature stages of medically important fly species, Puducherry, South India: a preliminary study.Frontiers in insect science · 2025Article
- Article
- Review
- Article
- Evidence of two mitochondrial lineages and genetic variability in forensically important Lucilia eximia (Diptera: Calliphoridae) in Colombia.Journal of medical entomology · 2023Article
- Characterizing Billbug (Insects · 2021Article
- Review
- Testing the Accuracy of Vegetation-Based Ecoregions for Predicting the Species Composition of Blow Flies (Diptera: Calliphoridae).Journal of insect science (Online) · 2021Article
- Influence of montane altitudinal ranges on species distribution models; evidence in Andean blow flies.PeerJ · 2020Article
- Ecological and geographical speciation inInternational journal for parasitology. Parasites and wildlife · 2019Article
- New Distribution Record for Lucilia cuprina (Diptera: Calliphoridae) in Indiana, United States.Journal of insect science (Online) · 2018Article
- Do longer sequences improve the accuracy of identification of forensically important Calliphoridae species?PeerJ · 2018Article
- Article
- Integrative taxonomy of the genusPeerJ · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Correct identification of forensically important insects, such as flies in the family Calliphoridae, is a crucial step for them to be used as evidence in legal investigations. Traditional identification based on morphology has been effective, but has some limitations when it comes to identifying immature stages of certain species. DNA-barcoding, using COI, has demonstrated potential for rapid and accurate identification of Calliphoridae, however, this gene does not reliably distinguish among some recently diverged species, raising questions about its use for delimitation of species of forensic importance. To facilitate DNA based identification of Calliphoridae in the Caribbean we developed a vouchered reference collection from across the region, and a DNA sequence database, and further added the nuclear ITS2 as a second marker to increase accuracy of identification through barcoding. We morphologically identified freshly collected specimens, did phylogenetic analyses and employed several species delimitation methods for a total of 468 individuals representing 19 described species. Our results show that combination of COI + ITS2 genes yields more accurate identification and diagnoses, and better agreement with morphological data, than the mitochondrial barcodes alone. All of our results from independent and concatenated trees and most of the species delimitation methods yield considerably higher diversity estimates than the distance based approach and morphology. Molecular data support at least 24 distinct clades within Calliphoridae in this study, recovering substantial geographic variation for
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.