ArticleScientific reports2017
Identification of reference genes for qRT-PCR in granulosa cells of healthy women and polycystic ovarian syndrome patients.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Effects of Testosterone on Cumulus Cells Gene Expression in Patients with Diminished Ovarian Reserve.Reproductive sciences (Thousand Oaks, Calif.) · 2025Trial
- Late-follicular androgen and estrogen rise associated with clinical pregnancy in IVF/ICSI with granulosa cell studies.Reproduction & fertility · 2026Article
- Use of low versus high oxygen tension during pre-maturation only in capacitation in vitro maturation (CAPA-IVM): impact on oocyte maturation and metabolism.Journal of assisted reproduction and genetics · 2026Article
- Functional characterization of a bovine luteal cell culture model: Effects of passage number.PloS one · 2025Article
- Transcriptome profiling reveals dysregulation of inflammatory and protein synthesis genes in PCOS.Scientific reports · 2024Article
- Article
- Genetic and Epigenetic Profiles of Polycystic Ovarian Syndrome and In Vitro Bisphenol Exposure in a Human Granulosa Cell Model.Biomedicines · 2024Article
- A Longitudinal Study of the Relationship of Adiponectin with Reproduction in Infertile Women Undergoing IVF/ICSI Treatment, and an Experimental Study in Human Granulosa Cells.Life (Basel, Switzerland) · 2023Article
- PPIA, HPRT1, and YWHAZ are suitable reference genes for quantitative polymerase chain reaction assay of the hypothalamic-pituitary-gonadal axis in sows.Animal bioscience · 2022Article
- Article
- Single-Oocyte Gene Expression Suggests That Curcumin Can Protect the Ovarian Reserve by Regulating the PTEN-AKT-FOXO3a Pathway.International journal of molecular sciences · 2021Article
- Roles of kisspeptin in IVF/ICSI-treated infertile women and in human granulosa cells.Experimental biology and medicine (Maywood, N.J.) · 2021Article
- ADAMTS1 and HSPG2 mRNA levels in cumulus cells are related to human oocyte quality and controlled ovarian hyperstimulation outcomes.Journal of assisted reproduction and genetics · 2020Article
- Genome-wide methylation profiling in granulosa lutein cells of women with polycystic ovary syndrome (PCOS).Molecular and cellular endocrinology · 2020Article
- The Acetylation of Lysine-376 of G3BP1 Regulates RNA Binding and Stress Granule Dynamics.Molecular and cellular biology · 2019Article
- Systematic Analysis of Intestinal MicroRNAs Expression in HCC: Identification of Suitable Reference Genes in Fecal Samples.Frontiers in genetics · 2019Article
- Altered expression of the kisspeptin/KISS1R and neurokinin B/NK3R systems in mural granulosa and cumulus cells of patients with polycystic ovarian syndrome.Journal of assisted reproduction and genetics · 2019Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Comparative gene expression analysis by qRT-PCR is commonly used to detect differentially expressed genes in studies of PCOS pathology. Impaired GC function is strongly associated with PCOS pathogenesis, and a growing body of studies has been dedicated to identifying differentially expressed genes in GCs in PCOS patients and healthy women by qRT-PCR. It is necessary to validate the expression stability of the selected reference genes across the tested samples for target gene expression normalization. We examined the variability and stability of expression of the 15 commonly used reference genes in GCs from 44 PCOS patients and 45 healthy women using the GeNorm, BestKeeper, and NormFinder statistical algorithms. We combined the rankings of the three programs to produce a final ranking based on the geometric means of their stability scores. We found that HPRT1, RPLP0, and HMBS out of 15 examined commonly used reference genes are stably expressed in GCs in both controls and PCOS patients and can be used for normalization in gene expression profiling by qRT-PCR. Future gene-expression studies should consider using these reference genes in GCs in PCOS patients for more accurate quantitation of target gene expression and data interpretation.
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