Evidence map›Paper›PMID 28761104›Full record

ArticleScientific reports2017

Microfluidic sorting of intrinsically magnetic cells under visual control.

Ahne Myklatun, Michele Cappetta, Michael Winklhofer, Vasilis Ntziachristos, Gil G Westmeyer

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.3field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 38 citations in OpenAlex.

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  12. Single-Cell Microgels for Diagnostics and Therapeutics.Advanced functional materials · 2021
    Article
  13. Article
  14. Article
  15. Review
  16. Review
  17. High-Throughput Microfluidic Sorting of Live Magnetotactic Bacteria.Applied and environmental microbiology · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Ahne MyklatunInstitute of Biological and Medical Imaging, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, D-85764, Neuherberg, Germany.
Michele CappettaInstitute of Biological and Medical Imaging, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, D-85764, Neuherberg, Germany.
Michael WinklhoferInstitute for Biology and Environmental Sciences, University of Oldenburg, Carl-von-Ossietzky-Strasse 9-11, D-26129, Oldenburg, Germany.ORCID 0000-0003-1352-9723
Vasilis NtziachristosInstitute of Biological and Medical Imaging, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, D-85764, Neuherberg, Germany.
Gil G WestmeyerInstitute of Biological and Medical Imaging, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, D-85764, Neuherberg, Germany. gil.westmeyer@tum.de.
Helmholtz Zentrum München · DETUM Klinikum · DECarl von Ossietzky Universität Oldenburg · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Magnetic cell sorting provides a valuable complementary mechanism to fluorescent techniques, especially if its parameters can be fine-tuned. In addition, there has recently been growing interest in studying naturally occurring magnetic cells and genetic engineering of cells to render them magnetic in order to control molecular processes via magnetic fields. For such approaches, contamination-free magnetic separation is an essential capability. We here present a robust and tunable microfluidic sorting system in which magnetic gradients of up to 1700 T/m can be applied to cells flowing through a sorting channel by reversible magnetization of ferrofluids. Visual control of the sorting process allowed us to optimize sorting efficiencies for a large range of sizes and magnetic moments of cells. Using automated quantification based on imaging of fluorescent markers, we showed that macrophages containing phagocytosed magnetic nanoparticles, with cellular magnetic dipole moments on the order of 10 fAm

Identifiers

PMID28761104
PMCPMC5537260
OpenAlexW2736413192

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.