ArticleScientific reports2017
Membrane Perfusion of Hydrophobic Substances Around Channels Embedded in the Contact Bubble Bilayer.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Programmable Lipid Bilayer Tension-Control Apparatus for Quantitative Mechanobiology.ACS nano · 2024Article
- Asymmetric Lipid Bilayers and Potassium Channels Embedded Therein in the Contact Bubble Bilayer.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Nanopore Filter: A Method for Counting and Extracting Single DNA Molecules Using a Biological Nanopore.Analytical chemistry · 2023Article
- Vectorial insertion of a β-helical peptide into membrane: a theoretical study on polytheonamide B.Biophysical journal · 2021Article
- Hysteresis of a Tension-Sensitive KJACS Au · 2021Article
- Physical and Chemical Interplay Between the Membrane and a Prototypical Potassium Channel Reconstituted on a Lipid Bilayer Platform.Frontiers in molecular neuroscience · 2021Review
- A Lipid-Bilayer-On-A-Cup Device for Pumpless Sample Exchange.Micromachines · 2020Article
- Constitutive boost of a KProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Thylakoid membranes contain a non-selective channel permeable to small organic molecules.The Journal of biological chemistry · 2018Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In fluidic biomembranes, lipids and membrane proteins diffuse restlessly, and lipid compositions change steadily. To mimic dynamic behavior of the biomembranes, a method for introducing rapid changes in the constituents in the lipid bilayer was developed. In contact bubble bilayers (CBB), as a water-in-oil droplet bilayer system, the bilayer hydrophobic interior is contiguous with the bulk oil phase. Making use of this geometrical feature as an access route, hydrophobic substances were administered into the bilayer. Polytheonamide B, a cytotoxic hydrophobic peptide, was applied, and oriented incorporation and relevant single-channel current recordings were enabled. Nystatin was pre-loaded in the CBB, and sterol perfusion exhibited slow development of the macroscopic current. On the contrary, the reconstituted KcsA potassium channels immediately attenuate the channel activity when cholesterol was applied. This oil-phase route in the CBB allows rapid perfusion of hydrophobic substances around the bilayer-embedded channels during continuous recordings of channel currents.
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Registered trials
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