ArticleScientific reports2017
Non-labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesions.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
- Advanced Microscopy Techniques.Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer · 2026Review
- Optical histopathology based on the nonlabeling analysis with multiphoton excitation imaging.Pathology international · 2025Review
- Towards next-generation diagnostic pathology: AI-empowered label-free multiphoton microscopy.Light, science & applications · 2024Review
- Current Landscape of Advanced Imaging Tools for Pathology Diagnostics.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2024Review
- Comprehensive Surface Histology of Fresh Resection Margins With Rapid Open-Top Light-Sheet (OTLS) Microscopy.IEEE transactions on bio-medical engineering · 2023Article
- Toward next-generation endoscopes integrating biomimetic video systems, nonlinear optical microscopy, and deep learning.Biophysics reviews · 2023Review
- Label-free multiphoton excitation imaging as a promising diagnostic tool for breast cancer.Cancer science · 2022Article
- Computer-Aided Detection of Quantitative Signatures for Breast Fibroepithelial Tumors Using Label-Free Multi-Photon Imaging.Molecules (Basel, Switzerland) · 2022Article
- High-Throughput, Label-Free and Slide-Free Histological Imaging by Computational Microscopy and Unsupervised Learning.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Novel Pixelwise Co-Registered Hematoxylin-Eosin and Multiphoton Microscopy Image Dataset for Human Colon Lesion Diagnosis.Journal of pathology informatics · 2022Article
- Large-scale, three-dimensional tissue cytometry of the human kidney: a complete and accessible pipeline.Laboratory investigation; a journal of technical methods and pathology · 2021Article
- Analysis on the Characterization of Multiphoton Microscopy Images for Malignant Neoplastic Colon Lesion Detection under Deep Learning Methods.Journal of pathology informatics · 2021Article
- Moxifloxacin based fluorescence imaging of intestinal goblet cells.Biomedical optics express · 2020Article
- In vivo non-invasive staining-free visualization of dermal mast cells in healthy, allergy and mastocytosis humans using two-photon fluorescence lifetime imaging.Scientific reports · 2020Article
- The Status of Advanced Imaging Techniques for Optical Biopsy of Colonic Polyps.Clinical and translational gastroenterology · 2020Review
- Deep-UV excitation fluorescence microscopy for detection of lymph node metastasis using deep neural network.Scientific reports · 2019Article
- Label-Free Multiphoton Endomicroscopy for Minimally Invasive In Vivo Imaging.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
- Three-Dimensional Holographic Electromagnetic Imaging for Accessing Brain Stroke.Sensors (Basel, Switzerland) · 2018Article
- Noninvasive diagnostic adjuncts for the evaluation of potentially premalignant oral epithelial lesions: current limitations and future directions.Oral surgery, oral medicine, oral pathology and oral radiology · 2018Review
- Tissue Intrinsic Fluorescence Spectra-Based Digital Pathology of Liver Fibrosis by Marker-Controlled Segmentation.Frontiers in medicine · 2018Article
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multiphoton excitation microscopy (MPM) is regarded as an effective tool that enables the visualization of deep regions within living tissues and organs, with little damage. Here, we report novel non-labeling MPM (NL-MPM) imaging of fresh human colorectal mucosa, which is useful for discriminating cancer lesions from normal tissues quantitatively without any need for resection, fixation, or staining. Using NL-MPM, we visualized three components in human colorectal mucosa, epithelial cells, immune cells, and basement membranes, based on their characteristic patterns of fluorescence. These patterns are characterized by the different auto-fluorescence properties of nicotinamide adenine dinucleotide, nicotinamide adenine dinucleotide phosphate, and flavin adenine dinucleotide and from second harmonic generation (SHG). NL-MPM images were at least as informative to pathologists as were 'conventional' images of fixed tissue sections stained with hematoxylin and eosin. Additionally, two quantitative parameters extracted from NL-MPM images - the nucleus diameter (index N) and the intensity of SHG in the basement membrane (index S) - rendered it possible to diagnose cancer regions effectively. In conclusion, NL-MPM is a novel, promising method for real-time clinical diagnosis of colorectal cancers, and is associated with minimal invasiveness.
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