Evidence map›Paper›PMID 28759636›Full record

ArticlePloS one2017

ALA-PpIX mediated photodynamic therapy of malignant gliomas augmented by hypothermia.

Carl J Fisher, Carolyn Niu, Warren Foltz, Yonghong Chen, Elena Sidorova-Darmos, James H Eubanks, Lothar Lilge

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed, 1 pooled it
2.1field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Carl J FisherDepartment of Medical Biophysics, University of Toronto, Toronto, ON, Canada.
Carolyn NiuDivision of Molecular Imaging, Princess Margaret Cancer Centre, Toronto, ON, Canada.
Warren FoltzDepartment of Radiation Oncology, University Health Network, Toronto, ON, Canada.
Yonghong ChenDivision of Molecular Imaging, Princess Margaret Cancer Centre, Toronto, ON, Canada.
Elena Sidorova-DarmosDepartment of Physiology, University of Toronto, Toronto, ON, Canada.
James H EubanksDepartment of Physiology, University of Toronto, Toronto, ON, Canada.
Lothar LilgeDepartment of Medical Biophysics, University of Toronto, Toronto, ON, Canada.ORCID http://orcid.org/0000-0001-5533-0005
University of Toronto · CAPrincess Margaret Cancer Centre · CAUniversity Health Network · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMalignant gliomas are highly invasive, difficult to treat, and account for 2% of cancer deaths worldwide. Glioblastoma Multiforme (GBM) comprises the most common and aggressive intracranial tumor. The study hypothesis is to investigate the modification of Photodynamic Therapy (PDT) efficacy by mild hypothermia leads to increased glioma cell kill while protecting normal neuronal structures.

methodsPhotosensitizer accumulation and PDT efficacy in vitro were quantified in various glioma cell lines, primary rat neurons, and astrocytes. In vivo studies were carried out in healthy brain and RG2 glioma of naïve Fischer rats. Hypothermia was induced at 1 hour pre- to 2 hours post-PDT, with ALA-PpIX accumulation and PDT treatments effects on tumor and normal brain PDT quantified using optical spectroscopy, histology, immunohistochemistry, MRI, and survival studies, respectively.

findingsIn vitro studies demonstrated significantly improved post-PDT survival in primary rat neuronal cells. Rat in vivo studies confirmed a neuroprotective effect to hypothermia following PpIX mediated PDT by T2 mapping at day 10, reflecting edema/inflammation volume reduction. Mild hypothermia increased PpIX fluorescence in tumors five-fold, and the median post-PDT rat survival time (8.5 days normothermia; 14 days hypothermia). Histology and immunohistochemistry show close to complete cellular protection in normal brain structures under hypothermia.

conclusionsThe benefits of hypothermia on both normal neuronal tissue as well as increased PpIX fluorescence and RG2 induced rat survival strongly suggest a role for hypothermia in photonics-based surgical techniques, and that a hypothermic intervention could lead to considerable patient outcome improvements.

Indexed as

Aminolevulinic AcidAnimalsAnimals, NewbornAstrocytesBrainBrain NeoplasmsCell Line, TumorCell SurvivalDrug Screening Assays, AntitumorFemaleGliomaHumansHypothermia, InducedMagnetic Resonance ImagingMaleNeuronsAminolevulinic AcidPhotosensitizing Agentsprotoporphyrin IXProtoporphyrins

Identifiers

PMID28759636
PMCPMC5536352
OpenAlexW2739877111

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.