ArticlePLoS genetics2017
Dynactin binding to tyrosinated microtubules promotes centrosome centration in C. elegans by enhancing dynein-mediated organelle transport.
Article in PLoS genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 48 citations in OpenAlex.
- Nuclear envelope buffering of cytoskeletal force stabilizes chromosome interactions inbioRxiv : the preprint server for biology · 2026Article
- Recombinant monoclonal antibodies for labeling tubulin post-translational modifications inmicroPublication biology · 2026Article
- Asymmetric partitioning of persistent paternal mitochondria during cell divisions safeguards embryo development and mitochondrial inheritance.Developmental cell · 2025Article
- ZYG-12/Hook's dual role as a dynein adaptor for early endosomes and nuclei is regulated by alternative splicing of its cargo binding domain.Molecular biology of the cell · 2025Article
- Cytoplasmic flow is a cell size sensor that scales anaphase.Nature cell biology · 2025Article
- Article
- Dynein directs prophase centrosome migration to control the stem cell division axis in the developing Caenorhabditis elegans epidermis.Genetics · 2024Article
- Nuclear-enriched protein phosphatase 4 ensures outer kinetochore assembly prior to nuclear dissolution.The Journal of cell biology · 2023Article
- Dynactin 2 acts as an oncogene in hepatocellular carcinoma through promoting cell cycle progression.Liver research (Beijing, China) · 2022Article
- The Expression and Function of Tubulin Isotypes inFrontiers in cell and developmental biology · 2022Review
- The coordination of spindle-positioning forces during the asymmetric division of the Caenorhabditis elegans zygote.EMBO reports · 2021Article
- Parthenolide Destabilizes Microtubules by Covalently Modifying Tubulin.Current biology : CB · 2021Article
- The Tubulin Code in Mitosis and Cancer.Cells · 2020Review
- The Tubulin Code in Microtubule Dynamics and Information Encoding.Developmental cell · 2020Review
- Activation and Regulation of Cytoplasmic Dynein.Trends in biochemical sciences · 2020Review
- Article
- DNA methylation biomarkers for nasopharyngeal carcinoma.PloS one · 2020Article
- Cytoskeleton Force Exertion in Bulk Cytoplasm.Frontiers in cell and developmental biology · 2020Review
- The Generation of Dynein Networks by Multi-Layered Regulation and Their Implication in Cell Division.Frontiers in cell and developmental biology · 2020Review
- A transient helix in the disordered region of dynein light intermediate chain links the motor to structurally diverse adaptors for cargo transport.PLoS biology · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The microtubule-based motor dynein generates pulling forces for centrosome centration and mitotic spindle positioning in animal cells. How the essential dynein activator dynactin regulates these functions of the motor is incompletely understood. Here, we dissect the role of dynactin's microtubule binding activity, located in the p150 CAP-Gly domain and an adjacent basic patch, in the C. elegans zygote. Analysis of p150 mutants engineered by genome editing suggests that microtubule tip tracking of dynein-dynactin is dispensable for targeting the motor to the cell cortex and for generating robust cortical pulling forces. Instead, mutations in p150's CAP-Gly domain inhibit cytoplasmic pulling forces responsible for centration of centrosomes and attached pronuclei. The centration defects are mimicked by mutations of α-tubulin's C-terminal tyrosine, and both p150 CAP-Gly and tubulin tyrosine mutants decrease the frequency of early endosome transport from the cell periphery towards centrosomes during centration. Our results suggest that p150 GAP-Gly domain binding to tyrosinated microtubules promotes initiation of dynein-mediated organelle transport in the dividing one-cell embryo, and that this function of p150 is critical for generating cytoplasmic pulling forces for centrosome centration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.