ArticleMetabolites2017
Non-Targeted Metabolomics Analysis of Golden Retriever Muscular Dystrophy-Affected Muscles Reveals Alterations in Arginine and Proline Metabolism, and Elevations in Glutamic and Oleic Acid In Vivo.
Article in Metabolites, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- Beta-hydroxy-beta-methylbutyrate (HMB) improves daily activity and whole-body protein metabolism in Duchenne muscular dystrophy dogs: a pilot study.Scientific reports · 2025Article
- Integrated metabolomics and intestinal microbiota analysis to reveal anti-post-weaning diarrhea mechanisms of Modified Yupingfeng Granule in Rex rabbits.Frontiers in microbiology · 2025Article
- Characterization of disease-specific alterations in metabolites and effects of mesenchymal stromal cells on dystrophic muscles.Frontiers in cell and developmental biology · 2024Article
- Effects of leucism on organ development and molecular mechanisms in Northern snakehead (Channa argus) beyond pigmentation alterations.Scientific reports · 2023Article
- Biomarkers for Duchenne muscular dystrophy progression: impact of age in the mdx tongue spared muscle.Skeletal muscle · 2023Article
- Integrating (Nutri-)Metabolomics into the One Health Tendency-The Key for Personalized Medicine Advancement.Metabolites · 2023Review
- Biomarkers for Duchenne muscular dystrophy progression: impact of age in the mdx tongue spared muscle.Research square · 2023Article
- Myoblast-derived exosomes promote the repair and regeneration of injured skeletal muscle in mice.FEBS open bio · 2022Article
- Biomarkers in Duchenne Muscular Dystrophy.Current heart failure reports · 2022Review
- Multi Platforms Strategies and Metabolomics Approaches for the Investigation of Comprehensive Metabolite Profile in Dogs withInternational journal of molecular sciences · 2022Article
- Multiomics analysis of the mdx/mTR mouse model of Duchenne muscular dystrophy.Connective tissue research · 2021Article
- Article
- Metabolomics in the study of spontaneous animal diseases.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2020Review
- Longitudinal metabolomic analysis of plasma enables modeling disease progression in Duchenne muscular dystrophy mouse models.Human molecular genetics · 2020Article
- Metabolomic study of soft corals from the Colombian Caribbean: PSYCHE andScientific reports · 2020Article
- Canine metabolomics advances.Metabolomics : Official journal of the Metabolomic Society · 2020Review
- Disease-specific and glucocorticoid-responsive serum biomarkers for Duchenne Muscular Dystrophy.Scientific reports · 2019Article
- Metabolomics Analysis of Skeletal Muscles from FKRP-Deficient Mice Indicates Improvement After Gene Replacement Therapy.Scientific reports · 2019Article
- Metabolomes of mitochondrial diseases and inclusion body myositis patients: treatment targets and biomarkers.EMBO molecular medicine · 2018Article
- Metabolomic Analyses Reveal Extensive Progenitor Cell Deficiencies in a Mouse Model of Duchenne Muscular Dystrophy.Metabolites · 2018Article
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundLike Duchenne muscular dystrophy (DMD), the Golden Retriever Muscular Dystrophy (GRMD) dog model of DMD is characterized by muscle necrosis, progressive paralysis, and pseudohypertrophy in specific skeletal muscles. This severe GRMD phenotype includes moderate atrophy of the biceps femoris (BF) as compared to unaffected normal dogs, while the long digital extensor (LDE), which functions to flex the tibiotarsal joint and serves as a digital extensor, undergoes the most pronounced atrophy. A recent microarray analysis of GRMD identified alterations in genes associated with lipid metabolism and energy production.
methodsWe, therefore, undertook a non-targeted metabolomics analysis of the milder/earlier stage disease GRMD BF muscle versus the more severe/chronic LDE using GC-MS to identify underlying metabolic defects specific for affected GRMD skeletal muscle.
resultsUntargeted metabolomics analysis of moderately-affected GRMD muscle (BF) identified eight significantly altered metabolites, including significantly decreased stearamide (0.23-fold of controls, p = 2.89 × 10
conclusionsThe identification of elevated BF oleic acid (a long-chain fatty acid) is consistent with recent microarray studies identifying altered lipid metabolism genes, while alterations in arginine and proline metabolism are consistent with recent studies identifying elevated L-arginine in DMD patient sera as a biomarker of disease. Together, these studies demonstrate muscle-specific alterations in GRMD-affected muscle, which illustrate previously unidentified metabolic changes.
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