ArticleThe Journal of general virology2017
Tree shrew, a potential animal model for hepatitis C, supports the infection and replication of HCV in vitro and in vivo.
Article in The Journal of general virology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 57 citations in OpenAlex.
- Review
- Fatty acid synthase promotes Hantaan virus infection through lipogenic regulation in the tree shrew model.Zoological research · 2026Article
- 1,4-dinitrosopiperazine specifically induces malignant nasopharyngeal transformation through the cytochrome P450 enzyme.Cancer cell international · 2026Article
- Natural Hepacivirus Infection in Tree Shrews: A Call for Routine Screening in Hepatitis Virus Research.Viruses · 2025Article
- Decoding the virome reveals diverse novel viruses in tree shrews (Tupaia belangeri) in Yunnan Province.Virologica Sinica · 2025Article
- Identification and characterization of the tumor necrosis factor receptor superfamily in the Chinese tree shrew (Tupaia belangeri chinensis).BMC genomics · 2025Article
- Decoding thymic development: a single-cell atlas of tree shrew immunity.Frontiers in immunology · 2025Article
- Marmosets as models of infectious diseases.Frontiers in cellular and infection microbiology · 2024Review
- Epstein Barr virus infection in tree shrews alters the composition of gut microbiota and metabolome profile.Virology journal · 2023Article
- Cell and Animal Models for SARS-CoV-2 Research.Viruses · 2022Review
- Differences of energy adaptation strategies inFrontiers in physiology · 2022Article
- Molecular cloning and characterization of NPC1L1 in the Chinese tree shrew (Tupaia belangeri chinensis).Molecular biology reports · 2021Article
- Tree Shrew as an Emerging Small Animal Model for Human Viral Infection: A Recent Overview.Viruses · 2021Review
- Comparing the hippocampal miRNA expression profiles of wild and domesticated Chinese tree shrews (Tupaia belangeri chinensis).BMC ecology and evolution · 2021Article
- Animal Models for COVID-19: Hamsters, Mouse, Ferret, Mink, Tree Shrew, and Non-human Primates.Frontiers in microbiology · 2021Review
- Recapitulating Zika Virus Infection in Vagina of Tree ShrewFrontiers in cellular and infection microbiology · 2021Article
- Establishment of Tree Shrew Animal Model for Kaposi's Sarcoma-Associated Herpesvirus (HHV-8) Infection.Frontiers in microbiology · 2021Article
- Tree Shrew Is a Suitable Animal Model for the Study of Epstein Barr Virus.Frontiers in immunology · 2021Article
- Adaptive Immune Response against Hepatitis C Virus.International journal of molecular sciences · 2020Review
- Hemogram study of an artificially feeding tree shrew (Tupaia belangeri chinensis).Experimental animals · 2020Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The tree shrew (Tupaia belangeri chinensis), a small animal widely distributed in Southeast Asia and southwest China, has the potential to be developed as an animal model for hepatitis C. To determine the susceptibility of the tree shrew to hepatitis C virus (HCV) infection in vitro and in vivo, a well-established HCV, produced from the J6/JFH1-Huh7.5.1 culture system, was used to infect cultured primary tupaia hepatocytes (PTHs) and tree shrews. The in vitro results showed that HCV genomic RNA and HCV-specific nonstructural protein 5A (NS5A) could be detected in the PTH cell culture from days 3-15 post-infection, although the viral load was lower than that observed in Huh7.5.1 cell culture. The occurrence of five sense mutations [S391A, G397A, L402F and M405T in the hypervariable region 1 (HVR1) of envelope glycoprotein 2 and I2750M in NS5B] suggested that HCV undergoes genetic evolution during culture. Fourteen of the 30 experimental tree shrews (46.7 %) were found to be infected, although the HCV viremia was intermittent in vivo. A positive test for HCV RNA in liver tissue provided stronger evidence for HCV infection and replication in tree shrews. The results of an immunohistochemistry assay also demonstrated the presence of four HCV-specific proteins (Core, E2, NS3/4 and NS5A) in the hepatocytes of infected tree shrews. The pathological changes observed in the liver tissue of infected tree shrews could be considered to be representative symptoms of mild hepatitis. These results revealed that the tree shrew can be used as an animal model supporting the infection and replication of HCV in vitro and in vivo.
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