Evidence map›Paper›PMID 28758530›Full record

ArticleInternational journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group2018

An optimised spectrophotometric assay for convenient and accurate quantitation of intracellular iron from iron oxide nanoparticles.

Mohammad Hedayati, Bedri Abubaker-Sharif, Mohamed Khattab, Allen Razavi, Isa Mohammed, Arsalan Nejad, Michele Wabler, Haoming Zhou, Jana Mihalic, Cordula Gruettner and 2 more

Open access · hybridAbstract read
In one paragraph

Article in International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
3.9field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 58 citations in OpenAlex.

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  16. HYPER: pre-clinical device for spatially-confined magnetic particle hyperthermia.International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Mohammad Hedayatia Department of Radiation Oncology and Molecular Radiation Sciences , Johns Hopkins University School of Medicine , Baltimore , MD , USA.
Bedri Abubaker-Sharifa Department of Radiation Oncology and Molecular Radiation Sciences , Johns Hopkins University School of Medicine , Baltimore , MD , USA.
Mohamed Khattaba Department of Radiation Oncology and Molecular Radiation Sciences , Johns Hopkins University School of Medicine , Baltimore , MD , USA.
Allen Razavia Department of Radiation Oncology and Molecular Radiation Sciences , Johns Hopkins University School of Medicine , Baltimore , MD , USA.
Isa Mohammeda Department of Radiation Oncology and Molecular Radiation Sciences , Johns Hopkins University School of Medicine , Baltimore , MD , USA.
Arsalan Nejada Department of Radiation Oncology and Molecular Radiation Sciences , Johns Hopkins University School of Medicine , Baltimore , MD , USA.
Michele Wablera Department of Radiation Oncology and Molecular Radiation Sciences , Johns Hopkins University School of Medicine , Baltimore , MD , USA.
Haoming Zhoua Department of Radiation Oncology and Molecular Radiation Sciences , Johns Hopkins University School of Medicine , Baltimore , MD , USA.
Jana Mihalicb Department of Environmental Health Sciences , Johns Hopkins Bloomberg School of Public Health , Baltimore , MD , USA.
Cordula Gruettnerc Micromod Partikeltechnologie GmbH , Rostock , Germany.
Theodore DeWeesea Department of Radiation Oncology and Molecular Radiation Sciences , Johns Hopkins University School of Medicine , Baltimore , MD , USA.
Robert Ivkova Department of Radiation Oncology and Molecular Radiation Sciences , Johns Hopkins University School of Medicine , Baltimore , MD , USA.
Johns Hopkins Medicine · USJohns Hopkins University · US

Funding

MEDICAL SCIENTIST TRAINING PROGRAMT32GM007309 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI COX, ANDREA L · 1985 to 2019
$43.2M
Enhancing Liver Cancer Treatment With Image-Guided Magnetic HyperthermiaR01CA194574 · NCI · JOHNS HOPKINS UNIVERSITY · PI IVKOV, ROBERT · 2015 to 2019
$3.2M
NCI NIH HHS R01 CA194574NIGMS NIH HHS T32 GM007309
6 · The paper itself

Abstract

We report the development and optimisation of an assay for quantitating iron from iron oxide nanoparticles in biological matrices by using ferene-s, a chromogenic compound. The method is accurate, reliable and can be performed with basic equipment common to many laboratories making it convenient and inexpensive. The assay we have developed is suited for quantitation of iron in cell culture studies with iron oxide nanoparticles, which tend to manifest low levels of iron. The assay was validated with standard reference materials and with inductively coupled plasma-mass spectrometry (ICP-MS) to accurately measure iron concentrations ∼1 × 10

Indexed as

Ferric CompoundsMetal NanoparticlesTriazinesBiological AssayCell Line, TumorColorimetryHumansIronMass SpectrometryNitric AcidSpectrophotometryFerene-SFerric Compoundsferric oxideIronNitric AcidTriazinesintracellular ironIron oxide nanoparticlesiron quantitation assaymass spectrometryUV/Vis spectrophotometry

Identifiers

PMID28758530
PMCPMC5871594
OpenAlexW2739495570

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.