Evidence map›Paper›PMID 28758130›Full record

ArticleJournal of diabetes research2017

Proinsulin Promotes Self-Renewal of a Hematopoietic Progenitor Cell Line In Vitro.

Yuewen Han, Tingting Liu, Yunding Zou, Ling Ji, Yuanyuan Li, Jing Li, Jing Wang, Guopin Chen, Jieping Chen, Liang Chen and 1 more

Open access · goldAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of diabetes research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Yuewen HanInstitute of Tropical Medicine, Third Military Medical University, Chongqing, China.
Tingting LiuInstitute of Tropical Medicine, Third Military Medical University, Chongqing, China.
Yunding ZouInstitute of Tropical Medicine, Third Military Medical University, Chongqing, China.ORCID 0000-0001-8069-7909
Ling JiInstitute of Tropical Medicine, Third Military Medical University, Chongqing, China.
Yuanyuan LiBiomedical Analysis Center, Third Military Medical University, Chongqing, China.
Jing LiInstitute of Tropical Medicine, Third Military Medical University, Chongqing, China.ORCID 0000-0003-0329-5337
Jing WangInstitute of Tropical Medicine, Third Military Medical University, Chongqing, China.
Guopin ChenBioengineering College, Chongqing University, Chongqing, China.
Jieping ChenDepartment of Hematology, Southwest Hospital, Chongqing, China.
Liang ChenDepartment of Plastic and Aesthetic Surgery, Southwest Hospital, Chongqing, China.
Zhijia YeInstitute of Tropical Medicine, Third Military Medical University, Chongqing, China.ORCID 0000-0002-9333-1604
Army Medical University · CNSouthwest Hospital · CNChongqing University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The objective of this study was to assess the effects of exogenously expressed proinsulin on the biological characters of a hematopoietic stem cell line (HSC) and erythroid myeloid lymphoid (EML) cells and explore new strategies for cell therapy for type I diabetes. EML cells were transduced with lentivirus particles carrying the human proinsulin (proINS) gene. The positive transduced cells were selected based on green fluorescence protein (GFP) positivity and puromycin resistance. Overexpression of proINS was confirmed via real-time PCR and Western blotting. The functional activity of the human proINS secreted by EML cells was elucidated by analyzing the activation of insulin receptor and its downstream signaling. Pro-INS + EML cells were able to prime the phosphorylation of insulin receptor as well as induce the expression of downstream genes of insulin receptor. Furthermore, Wnt3a can significantly promote self-renewal of Pro-INS + EML cells. However, we did not observe significant changes in the proliferation and differentiation of INS + EML cells, compared to the control EML cells. Our results might be useful for developing a new therapy for diabetes mellitus.

Indexed as

Cell DifferentiationCell ProliferationCell Self RenewalAntigens, CDCell LineErythroid Precursor CellsHematopoietic Stem CellsHumansPhosphorylationProinsulinReceptor, InsulinSignal TransductionTime FactorsTransduction, GeneticTransfectionUp-RegulationAntigens, CDINSR protein, humanProinsulinReceptor, InsulinWnt3A ProteinWNT3A protein, human

Identifiers

PMID28758130
PMCPMC5512049
OpenAlexW2729642377

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.