ArticleChemical science2016
Protein sensing in living cells by molecular rotor-based fluorescence-switchable chemical probes.
Article in Chemical science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 105 citations in OpenAlex.
- Multiobjective Fluorescent Molecule Design with a Data-Physics Dual-Driven Generative Framework.JACS Au · 2026Article
- A demethylation-activated fluorescent DNA aptamer strategy for visualising DNA alkylation repair in living cells.Chemical science · 2026Article
- Molecular rotor-based probes for protein monitoring in biomedical research.Nature reviews. Chemistry · 2026Review
- RiboBright reveals cell-type-specific differences in ribosome organization and movement.Nature communications · 2026Article
- Structure-function relationships in solvatochromic fluorophores targeting human and bovine carbonic anhydrases.Journal of inorganic biochemistry · 2025Article
- Emissive Alkylated Guanine Analogs as Probes for MonitoringACS omega · 2024Article
- Design of a palette of SNAP-tag mimics of fluorescent proteins and their use as cell reporters.Cell discovery · 2023Article
- Molecular rotors in haemoglobin and bovine serum albumin proteins.Journal of the Royal Society, Interface · 2022Article
- A small molecule antagonist of SMN disrupts the interaction between SMN and RNAP II.Nature communications · 2022Article
- Design and Synthesis of Fluorophore-Tagged Disparlure Enantiomers to Study Pheromone Enantiomer Discrimination in the Pheromone-Binding Proteins from the Gypsy Moth, Lymantria dispar.Journal of chemical ecology · 2022Article
- Excimer-monomer fluorescence changes by supramolecular disassembly for protein sensing and quantification.Chemical communications (Cambridge, England) · 2021Article
- A General Strategy to Control Viscosity Sensitivity of Molecular Rotor-Based Fluorophores.Angewandte Chemie (International ed. in English) · 2021Article
- AggFluor: Fluorogenic Toolbox Enables Direct Visualization of the Multi-Step Protein Aggregation Process in Live Cells.Journal of the American Chemical Society · 2020Article
- Visualizing and Manipulating Biological Processes by Using HaloTag and SNAP-Tag Technologies.Chembiochem : a European journal of chemical biology · 2020Review
- Ultrafast Oxime Formation Enables Efficient Fluorescence Light-up Measurement of DNA Base Excision.Journal of the American Chemical Society · 2019Article
- Article
- Fluorescent Probes of DNA Repair.ACS chemical biology · 2018Review
- Rational design of substituted maleimide dyes with tunable fluorescence and solvafluorochromism.Chemical communications (Cambridge, England) · 2018Article
- Aβ plaque-selective NIR fluorescence probe to differentiate Alzheimer's disease from tauopathies.Biosensors & bioelectronics · 2017Article
- A specific fluorescent probe reveals compromised activity of methionine sulfoxide reductases in Parkinson's disease.Chemical science · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this paper, we introduce a general design to construct fluorescence-switching probes by using conjugates of a fluorescent molecular rotor and protein specific ligands for the selective protein detection and real-time tracking of protein degradation in living cells. Upon the interaction of the ligand with the protein ligand-binding domain, the crowded surroundings restrict the bond rotation of the fluorescent molecular rotor to trigger the emission of a strong fluorescence signal, which is reduced upon the addition of a competitive ligand or after protein degradation. With this probe design, two fluorescent probes for MGMT and hCAII proteins were constructed and applied for detecting the endogenous proteins in living cells. In addition, real-time degradation kinetics of the alkylated-MGMT at the single living cell level were revealed for the first time. We believe that this fluorescence-switching probe design can possibly be extended for the analysis of other proteins, for which there are still no effective tools to visualize them in living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.