ArticleChemical science2016
A minimalist fluorescent probe for differentiating Cys, Hcy and GSH in live cells.
Article in Chemical science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The trial behind it
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Who cites it
15 citing papers in PubMed, 214 citations in OpenAlex.
- Single-Benzene-Based Clickable Fluorophores for In Vitro and In Vivo Bioimaging.ChemistrySelect · 2025Article
- γ-Glutamyltranspeptidase (GGT) Sensitive Fluorescence Probes for Cancer Diagnosis; Brief Review.Journal of fluorescence · 2024Review
- Rational design of a dual-reactive probe for imaging the biogenesis of both HRSC chemical biology · 2022Article
- Recent advances in activity-based probes (ABPs) and affinity-based probes (AChemical science · 2021Review
- A Simple Long-wavelength Fluorescent Probe for Simultaneous Discrimination of Cysteine/Homocysteine and Glutathione/Hydrogen Sulfide with Two Separated Fluorescence Emission Channels by Single Wavelength Excitation.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2020Article
- Giant single molecule chemistry events observed from a tetrachloroaurate(III) embedded Mycobacterium smegmatis porin A nanopore.Nature communications · 2019Article
- Exploring cysteine regulation in cancer cell survival with a highly specific "Lock and Key" fluorescent probe for cysteine.Chemical science · 2019Article
- A Fluorescent Probe Based on Pyrene Ring for Detecting Cys and its Application in Biology.Journal of fluorescence · 2019Article
- Visualization of oxidative injury in the mouse kidney using selective superoxide anion fluorescent probes.Chemical science · 2018Article
- Biodegradable nanoprobe based on MnOMikrochimica acta · 2018Article
- A fluorescence and UV/vis absorption dual-signaling probe with aggregation-induced emission characteristics for specific detection of cysteine.RSC advances · 2018Article
- A Facile Cyclization Method Improves Peptide Serum Stability and Confers Intrinsic Fluorescence.Chembiochem : a European journal of chemical biology · 2017Article
- Fluorescent Probes with Multiple Binding Sites for the Discrimination of Cys, Hcy, and GSH.Angewandte Chemie (International ed. in English) · 2017Review
- A multi-signal fluorescent probe for simultaneously distinguishing and sequentially sensing cysteine/homocysteine, glutathione, and hydrogen sulfide in living cells.Chemical science · 2017Article
- Quantification of cysteine hydropersulfide with a ratiometric near-infrared fluorescent probe based on selenium-sulfur exchange reaction.Chemical science · 2016Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A simple molecule, tetrafluoroterephthalonitrile (4F-2CN), was discovered to be an efficient fluorescent probe for detecting biological thiol species. The probe responded to Cys and emitted strong green fluorescence, whereas it reacted with Hcy/GSH and generated blue fluorescence. Addition of CTAB (cetyl trimethylammonium bromide) was observed to alter the fluorescence color of the reaction product of 4F-2CN and Hcy (from blue to green), but no alteration of the fluorescence color occurred for Cys and GSH. For the very first time, cell imaging experiments showed that the three commonly occurring thiols (Cys/Hcy/GSH) could be differentiated using a single fluorescent probe. In addition, the reaction product of 4F-2CN and Cys exhibits two-photon properties, offering a potentially useful tool for tissue imaging studies. To the best of our knowledge, 4F-2CN is currently the smallest fluorescent probe for thiol detection. We envision that this new and versatile probe will be a useful tool for further elucidating the roles of thiols in biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.