ArticleChemical science2015
Design, preparation, and selection of DNA-encoded dynamic libraries.
Article in Chemical science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Protocol for covalent ligand discovery via library-versus-proteome screening.STAR protocols · 2026Article
- Scaling covalent ligand discovery through dynamic combinatorial library-versus-proteome screening.Nature communications · 2026Article
- Toward the Chemoenzymatic Synthesis of DNA-Encoded Libraries.ACS central science · 2026Review
- Flexibility-tuning of dual-display DNA-encoded chemical libraries facilitates cyclic peptide ligand discovery.Nature communications · 2025Article
- Protein-templated ligand discovery via the selection of DNA-encoded dynamic libraries.Nature chemistry · 2024Article
- A DNA-encoded chemical library based on chiral 4-amino-proline enables stereospecific isozyme-selective protein recognition.Nature chemistry · 2023Article
- Trio-pharmacophore DNA-encoded chemical library for simultaneous selection of fragments and linkers.Nature communications · 2023Article
- Recent advances in DNA-encoded dynamic libraries.RSC chemical biology · 2022Review
- Converting Double-Stranded DNA-Encoded Libraries (DELs) to Single-Stranded Libraries for More Versatile Selections.ACS omega · 2022Article
- Exploring the Structural Diversity of DNA Bottlebrush Polymers Using an Oligonucleotide Macromonomer Approach.Macromolecules · 2022Article
- Strategies for developing DNA-encoded libraries beyond binding assays.Nature chemistry · 2022Review
- A mating mechanism to generate diversity for the Darwinian selection of DNA-encoded synthetic molecules.Nature chemistry · 2022Article
- Modular assembly and encoding strategies for dual-display DNA-encoded chemical libraries.Chemical communications (Cambridge, England) · 2021Article
- Scanning Protein Surfaces with DNA-Encoded Libraries.ChemMedChem · 2021Review
- Selection of DNA-encoded chemical libraries against endogenous membrane proteins on live cells.Nature chemistry · 2021Article
- Photocrosslinking of cDNA Display Molecules with Their Target Proteins as a New Strategy for Peptide Selection.Molecules (Basel, Switzerland) · 2020Article
- Expanding the materials space of DNA via organic-phase ring-opening metathesis polymerization.Chem · 2019Article
- Versatile protein recognition by the encoded display of multiple chemical elements on a constant macrocyclic scaffold.Nature chemistry · 2018Article
- Discovery of a Covalent Kinase Inhibitor from a DNA-Encoded Small-Molecule Library × Protein Library Selection.Journal of the American Chemical Society · 2017Article
- DNA-encoded chemistry: enabling the deeper sampling of chemical space.Nature reviews. Drug discovery · 2017Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report a method for the preparation and selection of DNA-encoded dynamic libraries (DEDLs). The library is composed of two sets of DNA-linked small molecules that are under dynamic exchange through DNA hybridization. Addition of the protein target shifted the equilibrium, favouring the assembly of high affinity bivalent binders. Notably, we introduced a novel locking mechanism to stop the dynamic exchange and "freeze" the equilibrium, thereby enabling downstream hit isolation and decoding by PCR amplification and DNA sequencing. Our DEDL approach has circumvented the limitation of library size and realized the analysis and selection of large dynamic libraries. In addition, this method also eliminates the requirement for modified and immobilized target proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.